【摘要】 目的探讨裙带菜多糖硫酸酯S-UPPSⅠB体外诱导人肝癌Hep G-2细胞凋亡的作用及机制。方法采用四甲基偶氮唑蓝（MTT）法检测S-UPPSⅠB对Hep G-2细胞增殖作用的影响;激光共聚焦显微镜测定Hep G-2细胞内[Ca²⁺]i水平;流式细胞仪测定细胞凋亡率及Bcl-2、Bax、Cyt-c、p53的表达;Caspase-3,-9试剂盒检测蛋白表达。结果 S-UPPSⅠB抑制Hep G-2细胞的IC₅₀值为50.09μg·m L^-1,随着给药剂量的增加,凋亡率亦增加,并且对Ca²⁺及其通路的相关蛋白有明显的调节作用,其中[Ca²⁺]i的水平及Cyt-C、Caspase-3,-9、p53的表达均有显著增加（P<0.05）,Bcl-2/Bax比值降低。结论裙带菜多糖硫酸酯S-UPPSⅠB可显著抑制人肝癌Hep G-2细胞增殖,并可通过启动线粒体途径诱导Hep G-2细胞发生凋亡。更多还原

【Abstract】 OBJECTIVE To investigate the mechanism of human liver cancer Hep G-2 cells apoptosis induced by Undaria pinnatifida sulfated polysaccharides S-UPPSⅠB.METHODS The anti-proliferation effects of S-UPPSⅠB on Hep G-2 cells was by MTT assay.^[Ca2+]i in Hep G-2 cells was detected by laser cofocal scaning microscopy（LCSM）.The apoptosis rate and protein expression level of Bcl-2,Bax,Cyt-C and p53 were detected by flow cytometry（FCM）.Caspase Assay Kit was used to detected the activities of Caspase-3 and-9.RESULTS S-UPPSⅠB could inhibit the proliferation of Hep G-2 cells and the IC₅₀was 50.09μg·m L^-1.With the increase of drug delivery dosage,apoptosis rate also increased,and the significant regulating effects of S-UPPSⅠB on Ca²⁺and its associated channel proteins were observed.The^[Ca2+]i level,the protein expression level of Cyt-c,p53 and the activities of Caspase-3 and-9 were all increased remarkably（P<0.05）.The ratio of Bcl-2 to Bax was reduced.CONCLUSIONUndaria pinnatifida sulfated polysaccharides S-UPPSⅠB can effectively inhibit the proliferation of human liver cancer Hep G-2 cells by inducing apoptosis of Hep G-2 cells through mitochondrial pathway.更多还原