【摘要】 目的合成铜类抗肿瘤金属药物并探讨其作用机制。方法合成了水杨醛氨基硫脲双核铜配合物[（CuⅡ）₂（L）₂（H₂O）（MeOH）]（NO₃^-）——2,利用X-ray单晶衍射、元素分析和红外光谱确证其结构。结果与结论该配合物对人肝癌细胞株BEL-7704具有很强的抑制活性,并且能使细胞周期停滞在S期。谷胱甘肽、活性氧、线粒体膜电位和蛋白质印迹实验结果显示该化合物是通过ROS介导的线粒体通路诱导癌细胞凋亡,同时伴随着Bcl-2家族蛋白的表达量的变化。更多还原

【Abstract】 One binuclear copper[Cu（Ⅱ）]complex of the Schiff base ligand（salicylaldehyde thiosemicarbazone,HL）,namely[（CuⅡ）₂（L）₂（H₂O）（MeOH）]（NO₃^-）₂,was synthesized and well characterized by single crystal X-ray diffraction,elemental analysis and infrared（IR）spectroscopic analysis.The Cu（Ⅱ）complex strongly promoted the apoptosis of BEL-7404 cells and had a capacity to arrest the cell cycle at S phase of the cells.Furthermore,glutathione/oxidized glutathione（GSH/GSSG）assay,reactive oxygen species（ROS）,mitochondrial membrane potential and western blot analyses revealed that the Cu（Ⅱ）complex exerts its cytotoxicity through an ROS-mediated intrinsic mitochondrial pathway accompanied by the regulation of Bcl-2 family proteins.更多还原