【摘要】 目的:研究莪术-三棱药对合煎液对大鼠子宫肌瘤的防治作用及机制。方法:将大鼠随机分为正常组、模型组、阳性组（米非司酮,2.25 mg/kg）和药对合煎液组（莪术-三棱药对合煎液,6.0 g/kg）,每组10只。除正常组外,其余各组大鼠分别于每周一、三、五im苯甲酸雌二醇注射液（0.5 mg/kg）,持续12周;从第13周开始,造模组大鼠增加im黄体酮注射液（5 mg/kg）,并每天ig给药1次,至实验第16周。给药结束后,测定大鼠子宫系数,苏木精-伊红染色观察大鼠子宫病理变化并测定平滑肌厚度,免疫组化染色法测定大鼠子宫组织中转化生长因子β₃（TGF-β₃）和基质金属蛋白酶11（MMP-11）蛋白表达。结果:与正常组比较,模型组大鼠子宫系数升高、子宫发生明显病理变化、子宫平滑肌厚度增加、子宫组织中TGF-β₃和MMP-11蛋白表达增强（P<0.05或P<0.01）;与模型组比较,阳性组和药对合煎液组大鼠上述变化均显著改善（P<0.05或P<0.01）。结论:莪术-三棱药对合煎液对大鼠子宫肌瘤有一定的防治作用,其机制可能与下调子宫组织中TGF-β₃、MMP-11蛋白表达有关。更多还原

【Abstract】 OBJECTIVE:To study the preventive and therapeutic effect and its mechanism of medicine pair decoction liquid of Curcuma phaeocaulis-Sparganium stoloniferum on rats with uterine myoma. METHODS:Rats were randomly divided into normal group,model group,positive group（mifepristone,2.25 mg/kg）and medicine pair decoction liquid group（C. phaeocaulis-S. stoloniferum decoction liquid,6.0 g/kg）,10 in each group. Except for the normal group,rats in other groups were injected Estradiol benzoate injection（0.5 mg/kg） intramuscularly every Monday,Wednesday and Friday,for 12 weeks. From the 13 th week,rats in modeling group were added Progesterone injection（5 mg/kg）intramuscularly as well as relevant medicines intragastrically,once a day,until the 16 th week. After administration,uterine coefficient of rats was detected. HE staining was used to observe the pathological changes of uterus and determine the thickness of smooth muscle;immunohistochemical staining was adopted to detect the transforming growth factor β₃（TGF-β₃）,matrix metalloproteinase 11（MMP-11）protein expressions in uterus tissue of rats. RESULTS:Compared with normal group,uterine coefficient was increased in model group,pathological changes were obvious in uterus,thickness of smooth muscle was increased,TGF-β₃and MMP-11 protein expressions were enhanced（P<0.05 or P<0.01）.Compared with model group,above-mentioned changes were improved significantly in positive group and medicine pair decoction liquid groups（P<0.05 or P<0.01）. CONCLUSIONS:Medicine pair decoction liquid of C. phaeocaulis-S. stoloniferum shows certain preventive and therapeutic effect on rats with uterine myoma. The mechanism may be associated with downregulating the TGF-β₃,MMP-11 protein expressions in uterus tissue.更多还原