【摘要】 目的:优化柴葛退热散超微粉碎工艺;比较超微粉和普通细粉中有效成分的含量和显微特征。方法:以柴葛退热散中3种有效成分(葛根素、甘草苷和黄芩苷)的含量以及粉末d(0.5)为主要指标,以堆密度、休止角及显微特征为参考指标,设计正交试验对超微粉碎工艺中粉末的初始粒径、水分及粉碎频率进行优化。比较超微粉与普通细粉(过65/80目筛)中3种有效成分的含量及显微镜下草酸钙针晶的观察结果。结果:超微粉优化工艺为选择初始粒径为过65目筛、水分为2.5%的药材粉末在粉碎频率为60 Hz下粉碎;验证试验中3份超微粉样品的d(0.5)平均值为31.5μm(RSD=0.45%,n=3),其中葛根素、甘草苷和黄芩苷的含量分别为0.232、0.212、8.962 mg/g(RSD分别为1.31%、1.62%、0.89%,n=3),比普通细粉中3种有效成分含量(0.158、0.160、6.140mg/g)约高30%~40%。超微粉中未见或仅少量可见普通细粉中常见的成束草酸钙针晶。结论:优化的超微粉碎工艺稳定、可行;柴葛退热散超微粉中3种有效成分的含量高于普通粉中含量,且因草酸钙针晶的减少有可能减少其临床不良反应。更多还原

【Abstract】 OBJECTIVE:To optimize the ultrafine pulverization technology in Chaige tuire powder;to compare the content and microcharacteristics between ultrafine powder and ordinary powder. METHODS:Using contents of 3 active ingredients(puerarin,glycyrrhizin and baicalin)and powder d(0.5)as main indexes,bulk density,angle of repose and microcharacteristic as reference indexes,orthogonal test was designed to optimize the initial particle size,moisture and pulverized frequency in ultrafine pulverization technology. Contents of 3 active ingredients of ultrafine powder and ordinary powder(over 65/80 mesh sieve)and observation results of calcium oxalate crystal under microscope were compared. RESULTS:The optimized technology was as follow as over 65 mesh sieve,moisture of 2.5% preliminary powder in 60 Hz of frequency for pulverization. In verification test,d(0.5)average value of 3 ultrafine powder samples was 31.5 μm(RSD=0.45%,n=3);contents of puerarin,glycyrrhizin and baicalin were0.232 mg/g(RSD=1.31%,n=3),0.212 mg/g(RSD=1.62%,n=3),8.962 mg/g(RSD=0.89%,n=3),respectively,which were increased about 30%-40% than in ordinary powder(0.158,0.156 69,6.140 mg/g). There were no or little bundles of calcium oxalate crystal that is common in ordinary powder. CONCLUSIONS:Optimized ultrafine pulverization technology is stable and feasible;contents of 3 active ingredients in Chaige tuire ultrafine powder are higher and calcium oxalate crystal are litter than ordinary powder,which possibly reduces the adverse reactions in clinical application.更多还原