【摘要】 目的:比较液相色谱-串联质谱（LC-MS/MS）法与酶放大免疫（EMIT）法测定人血清中丙戊酸（VPA）浓度的差异,为临床治疗药物监测提供参考。方法:分别采用LC-MS/MS法和EMIT法对144份住院或门诊患者血清样品中的VPA浓度进行检测,采用配对t检验、Pearson相关性分析和Bland-Altman偏差图等方法对其检测结果的差异进行评价。结果:LC-MS/MS法和EMIT法的检测结果呈正相关（r=0.924,P<0.05）;两者的回归方程为c_EMIT=0.920 7c_LC-MS/MS-1.114 4（r=0.924）;LC-MS/MS法和EMIT法测得的VPA平均血药浓度分别为（49.9±21.2）、（54.9±21.3）μg/m L,组间比较差异有统计学意义（P<0.05）;EMIT法检测结果比LC-MS/MS法偏高8.3μg/mL,95%置信区间为（-13.6,18.7）。结论:LC-MS/MS法和EMIT法测定人血清中VPA的浓度相关性良好,但检测结果有一定的差异,建议同一患者长期监测应选用同一种方法。更多还原

【Abstract】 OBJECTIVE:To compare the difference in the concentration determination of valproic acid（VPA）in human serum by LC-MS/MS and EMIT. METHODS:Both LC-MS/MS and EMIT methods were applied to determine the serum concentration of VPA in 144 inpatients or outpatients. The paired t-test,Pearson correlation analysis,Bland-Altman deviation chart and other methods were used to evaluate the difference in the results of concentration determination. RESULTS:The results of LC-MS/MS method was positively correlated with that of EMIT method（r=0.924,P<0.05）;the regression equation of them was c_EMIT=0.920 7c_LC-MS/MS-1.114 4（r=0.924）. Average serum concentrations of VPA determined by LC-MS/MS and EMIT were（49.9±21.2）and（54.9±21.3）μg/m L,with statistical significance（P<0.05）. The serum concentration of VPA determined by EMIT was higher than that by LC-MS/MS8.3 μg/m L,95% confidence interval was（-13.6,18.7）. CONCLUSIONS:The serum concentration of VPA determined by LC-MS/MS and EMIT have high correlation. But the determination results still have certain difference,it is suggested to use same method for longterm monitering.更多还原