【摘要】 为探讨非洲鸵鸟B淋巴细胞刺激因子(BAFF)蛋白对B淋巴细胞的作用,本研究根据前期克隆获得的非洲鸵鸟BAFF基因序列设计特异性引物,构建阳性重组表达质粒p ET-Os BAFF。将获得的表达产物进行SDS-PAGE电泳分析、western blot验证并利用MTT法检测其对于法氏囊B淋巴细胞的增殖作用。结果显示非洲鸵鸟BAFF重组融合蛋白纯度高,非洲鸵鸟法氏囊B淋巴细胞生长情况与重组蛋白BAFF的浓度有明显的相关性,表明其具有促进法氏囊淋巴细胞增殖的作用。本研究为探讨非洲鸵鸟BAFF作为B淋巴细胞生长促进剂和免疫增强剂等潜在用途提供了实验依据。更多还原

【Abstract】 B cell activating factor(BAFF) belongs to the tumor necrosis factor(TNF) family, which plays a critical role in B cell survival, proliferation, maturation and immunoglobulin secretion. In this study, two primers Os-F and Os-R contained Eco R I and Hind III restriction sites were designed to amplify the soluble part of Os BAFF based on the complete Os BAFF sequence in African ostrich in our previous research. The results of SDS-PAGE and western blot analysis confirmed that the soluble fusion protein His-Os BAFF was efficiently expressed in E.coli Rosset(DE3) In vitro, purified Os BAFF was not only able to promote the survival rate of African ostrich bursal lymphocytes, but also able to co-stimulate proliferation of mouse splenic B cells. These findings indicated that Os BAFF played an important role in survival and proliferation of African ostrich bursal lymphocytes, which could provide valuable information for research on the immune system of African ostrich and Os BAFF could serve as a potential immunologic factor for enhancing immunological efficacy in African ostrich and other birds.更多还原