【摘要】 研究以从江香猪、杜×长×大外三元杂交猪及贵州宗地花猪为研究对象,采用混合DNA池结合直接测序技术筛选猪MEF2D基因5’UTR及第1外显子区SNPs位点;同时利用生物信息学软件分析SNPs位点对核心启动子区、Cp G岛和转录因子结合位点的影响。结果表明:在猪MEF2D基因5’UTR区共筛查到4个SNPs位点,分别为A-511G、T-453A、T-242G和T-180A;生物信息学软件预测发现T-453A和T-242G位点附近有重要转录因子结合位点消失和新位点生成,据此推测猪MEF2D基因5’UTR区的T-453A和T-242G位点对调控启动子功能元件可能存在重要影响。更多还原

【Abstract】 The Congjiang Xiangpigs, the commercial crossbred pigs as well as the Guizhou Zongdi Huapigs as research animals. were used to screen the gene MEF2 D of 5ˊUTR and the SNPs sites in part first exon region by using DNA pool and PCR direct sequencing. Variety bioinformatics softwares were used to analyse the SNPs sites how to affect the core region of the promoter, transcription factors binding sites and Cp G island. The results showed there were four SNPs sites in the 5ˊUTR regions of MEF2 D, including A-511 G, T-453 A, T-242 G and T-180 A. It was identified that various new transcription factors binding sites emerged and some significant transcription factors binding sites disappeared nearby the region at T-453 A and T-242 G site. So we speculated that T-453 A and T-242 G site locating MEF2 D ˊ 5ˊ UTR have a deep effect on regulating the functional element of promoter.更多还原