【摘要】 目的研究生活饮用水中损伤性非苛养菌的耐药性。方法采用增菌培养基和选择性培养基对某小区生活饮用水进行损伤菌分离,获得分离株后,利用16S rRNA基因测序法进行种属鉴定;采用Kirby-Bauer纸片扩散法进行细菌的抗菌药物敏感性试验。结果在分离的58株损伤性非苛养菌中,耐药菌占51.72%,包括肠球菌属、肠杆菌属、葡萄球菌属、不动杆菌属、假单胞菌属。其中80%的损伤菌表现出对两种及以上抗菌药物耐药。耐药菌对复方新诺明的耐药最为普遍,耐药率为63.33%;对磺胺甲噁唑的耐药次之,占43.33%。结论测试小区生活饮用水中的损伤性非苛养菌耐药性较为普遍,应加强关注其潜在的人群健康风险。更多还原

【Abstract】 Objective To investigate the antibiotic resistance of non-fastidous injured bacteria in tap water from a housing estate. Methods Injured bacteria in the collected tap water samples were recovered by enrichment broth and isolated by the selective media. Bacteria were identified based on 16 S rRNA gene. Antibiotic susceptibility testing was performed using the Kirby-Bauer disc diffusion method. Results Of 58 strains isolated non-fastidous injured bacteria,51. 72% were drug resistant bacteria,including Enterococcus,Enterobacter,Staphylococcus,Acinetobacter and Pseudomonas. 20% of them showed resistance against only one antibiotic,while the others showed resistance against at least two antibiotics. Trimethoprim/sulfamethoxazole resistance was the most prevalent( 63. 33%). Sulfamethoxazole resistance was also widely distributed( 43. 33%). Conclusion Non-fastidous injured bacteria shows resistance to antibiotics in tap water,suggesting they have the potential risk to population.更多还原