【摘要】 目的探讨虫草素（Cordycepin）对高糖介导大鼠肾小管上皮细胞p38MAPK通路及TGF-β₁表达的影响。方法体外培养大鼠近端肾小管上皮细胞株（NRK52E细胞株）,分为正常对照组（NG组）、高糖组（HG组）、高糖+虫草素组（HG+C组）。应用定量RT-PCR测定NRK52E p38MAPK及TGF-β₁m RNA的表达;采用Western印迹方法检测不同时间点p-p38MAPK、TGF-β₁蛋白的表达水平。结果与NG组比较,HG组NRK52E细胞的p38MAPK、TGF-β₁m RNA表达增多（P<0.01）;p-p38MAPK蛋白、TGF-β₁蛋白表达与m RNA表达趋势一致（P<0.05）;而虫草素可以显著抑制高糖介导的NRK52E细胞p38MAPK的活化及TGF-β₁的表达。结论虫草素可以抑制高糖介导大鼠肾小管上皮细胞p38MAPK活化及TGF-β₁表达。更多还原

【Abstract】 Objective To investigate effect of cordycepin on p38 MAPK pathway and TGF-β₁ express in NRK52 E cells stimulated by high glucose.Methods NRK52 E cells were cultured in the mediun with normal glucose concentration（group NG）,high glucose concentration（group HG） and high glucose concentration+cordycepin（group HG+C）.The expression of p38 MAPK and TGF-β₁ mRNA was measured by quantitative RT-PCR;the expression level of p-p38 MAPK and TGF-β₁ protein was detected by Western blot.Results The p38 MAPK and TGF-β₁ mRNA in NRK52 E cells were highly expressed in group HG compared with group NG（P<0.01）;the tendency of p-p38 MAPK and TGF-β₁ protein expressed was accordance with expression of m RNA.However,cordycepin can significantly inhibit the activation of p38 MAPK pathway and TGF-β₁express in NRK52 E cells stimulated by high glucose.Conclusion Cordycepin can significantly inhibit the activation of p38 MAPK pathway and TGF-β₁ express in NRK52E cells stimulated by high glucose.更多还原