【摘要】 采用乙醇浸提法提取胡椒中的胡椒碱,通过单因素和L₁₆（4⁵）正交实验,确定了最佳提取工艺条件:提取温度为65℃,乙醇浓度为75%,料液比为1∶30,提取时间为0.5h,提取次数为3次。通过静态和动态实验,确定了大孔树脂分离胡椒碱的最佳工艺条件:树脂种类为AB-8,上柱浸提液浓度为1.1mg/mL,pH值为6,洗脱液为体积分数75%、pH值为5的乙醇溶液,胡椒碱纯度由浸提液的16.46%上升到69.32%。最后,通过C₁₈层析柱对大孔树脂纯化的胡椒碱粗品进一步纯化,用90%乙醇洗脱,经HPLC测定纯化后粗品中的胡椒碱纯度上升为81.26%。更多还原

【Abstract】 The piperine is extracted from pepper with ethanol extraction method,and the optimal extraction conditions are determined by single factor and L₁₆（4⁵）orthogonal experiments:the ethanol concentration of 75%,the extraction temperature of 65 ℃,the solid-liquid ratio of 1∶30,the extraction time of 0.5,the extraction times of 3.Based on the static and dynamic experiments,the optimal purification conditions for piperine separated by macroporous resins are obtained as follows:the type of macroporous resins of AB-8,the extract concentration of 1.1mg/mL and the pH of 6,the eluted liquid of 75%（V/V）ethanol solution and pH of 5,the purity of piperine increases from 16.46%to 69.32%.Finally,the crude product of piperine is purified by C₁₈ column,and eluted by 90%（V/V）ethanol solution,and the purity of piperine increases to 81.26% measured by HPLC method.更多还原