【摘要】 为了探讨ERK信号转导通路对脑炎原虫性脑肉芽肿形成的影响,应用普通染色、特殊染色、免疫组织化学技术和Western-blot技术分别对脑炎原虫性脑肉芽肿进行观察,分析脑炎原虫性脑肉芽肿形成与信号转导通路中神经生长因子(NGF)、酪氨酸蛋白激酶(TPK)、丝裂源活化蛋白激酶(MAPK)和细胞外信号调节激酶(ERK)等四种关键性蛋白的关系。结果显示,所有患病家兔的脑组织中均有数量不等的脑肉芽肿和非化脓性脑炎变化;免疫组化染色显示,NGF、TPK、MAPK和ERK的表达在肉芽肿中的星形胶质细胞、血管内皮细胞和成纤维细胞中明显增高,呈强阳性反应,染成深棕色;用Western-blot检测,患病家兔脑组织中的NGF、TPK、MAPK和ERK等信号转导通路关键蛋白表丰度明显高于健康家兔。以上结果表明,ERK信号转导通路通过强大的级联反应参与了家兔脑炎原虫性脑肉芽肿的形成,在脑肉芽肿形成过程中促进星形胶质细胞、血管内皮细胞和成纤维细胞增殖。更多还原

【Abstract】 To study the cerebral granulomatous formation caused by Encephalitozoon cuniculi (E.cuniculi) through ERK signal transduction pathway,the common staining,special staining,immunohistochemistry and Western-blot were used to observe the morphological changes of cerebral granuloma by E.cuniculi.Moreover,analyze the relationship between the formation of cerebral granuloma and four kinds of key proteins,such as NGF,TPK,MAPK and ERK in ERK signal transduction pathway was investigated.The results showed that there were a number of cerebral granuloma and nonsuppurative encephalitis in all of sick rabbits.The expression of NGF,TPK,MAPK and ERK was obviously higher in astrocytes,vascular endothelia and fibroblasts of the cerebral granuloma.These cells showed strong positive reaction and were stained as dark brown by immunohistochemistry staining.The key protein abundance of signal transduction pathway in sick rabbit’s brain tissue was much higher than that in control rabbit’s by Western-blot.It was indicated that the ERK signal transduction pathway was activated in the process of forming cerebral granuloma by E.cuniculi and could facilitated the proliferation of astrocytes,endothlia and fibroblastformed cerebral granuloma via strong cascade reactions.更多还原