In this study we developed a direct TaqMan quantitative PCR(direct-q PCR) method using Taqman probes for detection of canine parvovirus(CPV), this method did not extract nucleic acids. Direct-q PCR method was established by constructing recombinant plasmid. The reaction condition, specificity and sensitivity of this method were optimized. Clinical samples were collected and used for verification of this method. The result showed that the method had good specificity, with no amplification of common viruses i...
titative PCR and direct-q PCR both reached to 101copies/L,and the concentration of direct-q PCR pro-duction was 7 times than that of direct-q PCR,indicating that the process of nucleic acid extractionhas lost many nucleic acids.187 clinical materials were
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