【摘要】 目的:研究丁酸钠对β-乳球蛋白(β-Lg)致敏淋巴细胞的体外调节作用。方法:通过腹腔注射β-Lg,建立BALB/c小鼠牛乳过敏模型。造模成功后,分离致敏小鼠脾淋巴细胞与不同浓度的丁酸钠体外共同孵育,采用MTT法检测丁酸钠对致敏淋巴细胞生长的影响,ELISA法检测淋巴细胞上清液中总IgE和细胞因子(IFN-γ、IL-4、TNF-β、IL-6、IL-17、TGF-β)的含量,RT-PCR法检测淋巴细胞中G蛋白受体mRNA的表达。结果 :与过敏组比较,不同浓度丁酸钠均可有效抑制β-Lg过敏淋巴细胞的异常增殖,并显著下调致敏细胞分泌总IgE。同时,高浓度的丁酸钠对炎性细胞因子TNF-β、IL-17和IL-6有显著抑制作用,并显著促进TGF-β分泌(P<0.05)。丁酸钠还显著上调过敏淋巴细胞GPR41和GPR43受体表达,且呈剂量依赖效应。结论:丁酸钠通过结合G蛋白受体抑制致敏淋巴细胞的增殖并发挥其抗炎功能。更多还原

【Abstract】 [Objective] The aim of study was to investigate the effect of butyrate on the lymphocyte of β-lactoglobulin-sensitized mice in vitro. [Methods] BALB/c mice were intraperitoneally injected with β-Lactoglobulin(β-Lg), the sensitized splenocytes were stimulated in vitro with different doses of butyrate. The proliferation of splenocytes were tested by MTT colorimetry. The total Ig E and related cytokines(IFN-γ, IL-4, TNF-β, IL-6,TGF-β and IL-17) were detected by ELISA assay, the levels of GPR41 and GPR43 m RNA expressiom were measured by RT-PCR. [Results] Treatment with different doses of sodium butyrate significantly suppressed β-Lg induced proliferation of lymphocytes from sensitized mice and Ig E production. Furthermore, application of higher concentration of sodium butyrate markedly inhibited the production of IL-17, IL-6 and TNF-β, but increased the production of TGF-β when compared with the allergy group(P<0.05). Especially, sodium butyrate significantly up-regulated GPR41 and GPR43 receptor expression in a dose-dependent manner.[Conclusion] Sodium butyrate could inhibit the proliferation of sensitized lymphocytes and play anti-inflammation function through G protein receptor.更多还原