Flavonoids were extracted from rye seed by using alcohol.Alcohol concentration of 90% was ascertained on the basis of four single-factor experiments.The optimal extraction conditions,including alcohol volume,extraction temperature and extraction time,were investigated on the basis of three factors quadratic orthogonal design to analyze the major effect and mutual effect between two factors on the yield of flavonoids by the RSREG process of SAS procedure.The results indicated that the factor effect on the yi...