【摘要】 目的:探索玛咖提取物对运动耐力和脊神经元线粒体超微结构的影响。方法:50只Wistar大鼠随机分为5组。对照组:不进行游泳,灌胃等量蒸馏水;单纯游泳组:游泳,灌胃等量蒸馏水,玛咖提取物组(设4.0,5.3,8.0 g/kg 3个剂量组):游泳,给予相应剂量的玛咖提取物。游泳大鼠在游泳池内循环水流自由游泳,连续游泳并给药15 d,第16天游泳耐力测试后无痛处死大鼠,用投射电子显微镜观察脊髓神经元线粒体超微结构,放免法测定肌肉肌糖元、检测肌肉中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和游离钙水平。结果:与单纯游泳组比,沉下前游泳时间和游泳总时间分别延长了(%)19.83、60.28、77.55和55.34、73.91、94.47,差异均有统计学意义(P均<0.01);沉下次数分别减少了(%)34.35、51.18、57.96,差异有统计学意义(P<0.01);MDA和游离钙含量分别降低了(%)20.10、31.49、38.72,差异有统计学意义(P<0.01)和6.42、17.58、26.35;SOD、GSH-Px和肌糖原含量分别升高了(%)5.12、22.74、52.53、44.22、77.79、98.45,差异有统计学意义(P<0.01)和35.08、47.83、81.88,差异有统计学意义(P<0.01);脊神经元线粒体的体密度(Vd)、面密度(Sd)和数密度(Nd)分别减小了(%)7.79、18.18、31.17,16.95、27.34、43.31和13.51、23.19、43.15。结论:玛咖提取物具有保护脊髓神经元线粒体结构、抗氧化、增加肌糖原和提高运动能力等作用。更多还原

【Abstract】 Objective: To investigate the effects of maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance. Methods: The Wistar rats were randomly divided into 5 groups,including the control group（ no swimming）,the swimming group（ free swimming）,and 3 treatment groups treated with the maca extract at the doses of 4. 0,5. 3 and 8. 0 g/kg body weight. The animals in swimming and treatment groups were then for free swimming in the circulating water flow daily for 15 days. On the 16 thday after swimming endurance,the spinal and muscular tissues were collected from all groups. The mitochondrial ultrastructures of the neurons of the spinal cells were observed with the projection electron microscope,and the levels of the glycogen,malondialdehyde（ MDA）,superoxide dismutase（ SOD）,glutathione peroxidase（ GSH-Px）,and Ca²⁺ in muscle tissues were determined by the RIA method. Results: When rats were treated with maca extract（ at 4. 0,5. 3,8. 0 g/kg body weight）,the total swimming time and the swimming duration before sinking were increased by 19. 83%,60. 28%,77. 55%,and 55. 34%,73. 91%,94. 47%,respectively,compared with the simple swimming group（ P < 0. 01）,while the sinking times were decreased by 34. 35%,51. 18% and 57.96%,compared with those of the swimming group. Also,the levels of SOD,GSH-Px,and muscle glycogen in three treatment groups were enhanced by 5. 12%,22. 74%,52. 53%,44. 22%,77. 79%,98. 45%（ P < 0. 01）,and 35. 08%,47. 83%,81. 88%（ P <0. 01） respectively over the swimming rats without treatment,but the MDA content and the Ca2 +levels were reduced by 20. 10%,31.49% 38. 72%,and 6. 42%,17. 58%,26. 35%,compared with the simple swimming group（ P < 0. 01）. In addition,compared to the swimming group,the mitochondrial densities of volume（ Vd）,surface（ Sd） and numbers（ Nd） of spinal nerve cells in rats treated with maca extract（ 4. 0,5. 3,8. 0 g/kg body weight） were reduced by 7. 79%,18. 18%,31. 17%,16. 95%,27. 34%,43. 31%and 13. 51%,23. 19%,43. 15%,respectively. Conclusion: Our results demonstrated the protective effects of maca extract on the mitochondria of spinal cell and suggested that maca extract could improve the muscle antioxidant activity by increasing the levels of SOD,GSH-Px,and muscle glycogen.更多还原