【摘要】 为研究双氯芬酸钠对苏云金芽孢杆菌毒性的分子机制,使用基于iTRAQ的定量蛋白质组学技术为主要的研究方法,通过精确定量苏云金芽孢杆菌蛋白质含量并进行差异分析.本研究结果表明,双氯芬酸钠对苏云金芽孢杆菌的生长具有明显的抑制作用.分析得到了17个差异表达蛋白,其中有5个上调蛋白,主要参与脂肪酸生物合成、DNA和RNA的合成,12个下调蛋白,涉及氧化磷酸化、丙酮酸代谢、糖酵解途径、磷酸戊糖途径和氨基酸代谢等.功能分析显示,双氯芬酸钠通过影响细胞代谢过程、细胞组成和蛋白质催化等方式抑制苏云金芽孢杆菌生长.在差异表达蛋白相互作用网络中,RpoA、RplM、RplL、Tuf、InfA 5个蛋白连接度较高,属于网络中的关键节点,可能起着重要的调控作用.研究结果表明双氯芬酸钠的处理影响了苏云金芽孢杆菌多条代谢途径,干扰不同的生物过程,揭示了双氯芬酸钠对苏云金芽孢杆菌毒性的分子机制,为深入评价双氯芬酸钠对生态安全及人类健康的影响提供了重要的参考意义.更多还原

【Abstract】 To investigate the molecular toxicity of diclofenac sodium to Bacillus thuringiensis,the i TRAQ quantitative proteomics technique was used to identify and quantify the protein expression of this species.The results showed that diclofenac sodium had a significant inhibitory effect on the growth of B.thuringiensis.Seventeen proteins were differentially expressed.Among these proteins,5 up-regulated ones were mainly involved in fatty acid biosynthesis,DNA and RNA synthesis,while 12 down-regulated proteins were primarily associated with oxidative phosphorylation,pyruvate metabolism,glycolytic pathway,pentose phosphate pathway and amino acid metabolism.Functional analysis revealed that diclofenac inhibited the growth of B.thuringiensis by affecting cell metabolism,cellular composition and protein catalysis.In the interaction network of differentially expressed proteins,Rpo A,Rpl M,Rpl L,Tuf,Inf A were the key nodes in the network interacting closely for cellular regulation.The results indicated that diclofenac sodium could affect multiple metabolic pathways and interfere with different biological processes.These findings revealed the molecular toxicity of diclofenac sodium,and provided important references for the further evaluation of diclofenac sodium on the ecological security and human health.更多还原