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草地早熟禾PpGA2ox基因的克隆、亚细胞定位及表达分析

Cloning,Subcellular Localization and Expression Analysis of PpGA2ox Gene in Kentucky Bluegrass

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【作者】 檀鹏辉李俊陈小云张兰滕珂尹淑霞

【Author】 TAN Peng-hui;LI Jun;CHEN Xiao-yun;ZHANG Lan;TENG Ke;YIN Shu-xia;Turfgrass Research Institute,College of Forestry,Beijing Forestry University;Institute of Grassland Research,Chinese Academy of Agricultural Sciences;Shenzhen Guoyi Park Developments CO.LTD;

【机构】 北京林业大学林学院草坪研究所中国农业科学院草原研究所深圳市国艺园林建设有限公司

【摘要】 利用RACE技术从草地早熟禾中克隆赤霉素合成代谢的关键酶GA2-氧化酶基因(PpGA2ox),提交到GenBank,登录号为KX254272。其开放阅读框为1047bp,编码348个氨基酸。系统进化分析表明,PpGA2ox与大麦、小麦及二穗短柄草中的GA2ox亲缘关系较近。亚细胞定位结果显示,其编码的蛋白定位于细胞核和细胞质中。实时荧光定量分析表明,PpGA2ox在幼嫩叶片中的表达量最高,且受GA3、MeJA及IAA三种激素的诱导。

【Abstract】 To investigate the GA2-oxidase gene in Kentucky bluegrass,the PpGA2oxgene was cloned using RACE method in the study.The cDNA sequence was deposited in the NCBI database with accession number KX254272.The open reading frame of PpGA2ox gene was 1047 bp,corresponding to 348 amino acids.Phylogenetic analysis exhibited that the PpGA2ox protein was most closely related to those in barley,wheat and Brachypodium distachyon.Subcellular localization results proved that the PpGA2ox protein was localized in both the nucleus and cytoplasm.Real-time quantitative PCR analysis showed that PpGA2oxgene was expressed most abundantly in young leaves.Moreover,its expression could be induced by exogenous GA3,MeJA or IAA.

【基金】 国家自然科学基金项目“空间诱变草地早熟禾矮化突变体矮化机理研究”(31302016);深圳市科技计划项目“海绵型城市绿地建植关键技术研发”(JSGG20160229155434792)资助
  • 【文献出处】 中国草地学报 ,Chinese Journal of Grassland , 编辑部邮箱 ,2017年03期
  • 【分类号】Q943.2;S688.4
  • 【被引频次】4
  • 【下载频次】180
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