【摘要】 目的研究石斛属植物兜唇石斛Dendrobium aphyllum茎的化学成分。方法采用硅胶、凝胶、MCI、中压制备色谱(MPLC)和高效液相半制备色谱(semi-HPLC)等方法进行分离纯化,根据理化性质、核磁共振谱及质谱等方法鉴定化合物结构。结果从兜唇石斛的90%乙醇提取物中分离得到10个化合物,分别鉴定为4,7-二羟基-2-甲氧基-9,10-二氢菲(1)、2,4-二羟基-7-甲氧基-9,10-二氢菲(2)、2,5,7,9-四羟基-4-甲氧基-9,10-二氢菲(3)、4,7,9-三羟基-5-甲氧基-9,10-二氢菲(4)、松脂醇(5)、丁香酚-β-D-葡萄糖苷(6)、反式阿魏酸(7)、正十二烷醇(8)、β-谷甾醇(9)、β-胡萝卜苷(10)。结论化合物1~2、5~8为首次从该属植物中分离得到,化合物1~10均为首次在该植物中分离得到。更多还原

【Abstract】 Objective To study the chemical constituents from ethanol extracts of the stems of Dendrobium aphyllum. Methods Ten compounds were isolated by silica gel, Sephadex LH-20 column chromatography and reversed-phase semi-HPLC. Results Ten compounds were identified by analyzing their spectral data and comparing with the previously reported literatures as 4,7-dihydroxy-2-methoxy-9,10-dihydroxyphenanthrene(1), 2,4-dihydroxy-7-methoxy-9,10-dihydroxyphenanthrene(2), 4-methoxy-2,5,7,9-tetrahydroxy-9,10-dihydrophe-nanthrene(3), 5-methoxy-4,7,9-trihydroxy-9,10-dihydrophenanthrene(4), pinoresinol(5), eugenol-β-D-glucoside(6), trans-ferulic acid(7), dodecan-1-ol(8), β-sitosterol(9), and β-daucosterol(10). Conclusion All compounds are isolated from this plant for the first time.更多还原