【摘要】 目的采用UPLC-MS/MS法探讨甘草与麻黄配伍后对甘草中入血成分甘草酸、甘草次酸和甘草苷药动学的影响。方法将12只大鼠随机分成2组,各组分别灌胃给予单味甘草和麻黄-甘草（2∶1）水煎液后,于不同时间点采血,所得血药浓度-时间数据经DAS3.2.2计算相关的药动学参数。结果与单味甘草组相比,麻黄-甘草（2∶1）组甘草苷、甘草次酸和甘草酸的AUC_0-t（药-时曲线下面积）和C_max（最大血药浓度）均下降,MRT_0-t（平均驻留时间）和t_1/2z（血浆消除半衰期）均推迟,V_z/F（表观分布容积/吸收分数）均增大;甘草苷和甘草酸的CL_z/F（总体清除率/吸收分数）均增大;甘草酸的T_max（达峰时间）缩短了,甘草次酸的T_max则推迟了。结论甘草与麻黄配伍后,降低了甘草酸、甘草次酸和甘草苷的生物利用度,延缓了三者在体内的消除,增大了三者在体内的分布体积。更多还原

【Abstract】 AIM To investigate the effects of the compatibility of Ephedrae Herba （Mahuang） and Glycyrrhizae Radix et Rhizoma （Gancao） on pharmacokinetic changes of the plasma levels of glycyrrhizic acid,glycyrrhetinic acid and liquiritin by UPLC-MS/MS.METHODS Twelve SD rats were randomly divided into two groups and orally administered with Glycyrrhizae Radix et Rhizoma and Ephedrae Herba-Glycyrrhizae Radix et Rhizoma （2 ∶ 1）Decoction.Blood samples were collected at different time points,and the main pharmacokinetic parameters were calculated by DAS3.2.2 software.RESULTS Compared with Glycyrrhizae Radix et Rhizoma group,the AUC_0-t and Cmaxwere decreased,the MRT_0-tand t_1/2z were extended,the V_z/F was increased for glycyrrhizic acid,glycyrrhetinic acid and liquiritin; the CL_z/F was increased for glycyrrhizic acid and liquiritin; the Tmaxof glycyrrhizic acid was shortened and the Tmaxof glycyrrhetinic acid was extended.CONCLUSION After combining Glycyrrhizae Radix et Rhizoma and Ephedrae Herba,the bioavailability of glycyrrhizic acid,glycyrrhetinic acid and liquiritin are reduced,the elimination of them delays,and the distribution volume increases.更多还原