AIM: To evaluate the effect of exogenous hydrogen sulfide( H2S) from GYY4137 on lipophagy in mouse primary hepatocytes. METHODS: The C57BL/6 mouse primary hepatocytes isolated and cultured by 2-step in situ perfusion method were divided into 4 groups: the cells in control group were incubated with normal medium; the cells in model group were incubated with 1. 2 mmol/L oleic acid( OA) for 48 h; the cells in H2 S group or propargylglycine( PAG)group were incubated with 1. 2 mmol/L OA for 48 h followed by seru...