AIM:To study the roles of Toll-like receptor 4(TLR4) and TLR4 activator lipopolysaccharide(LPS) in colonic inflammation recovery.METHODS:Normal intestinal epithelial cells were cultured with LPS in vitro.The subgroups of the intestinal epithelial cells with differential expression of TLR4(low,normal and high) were constructed by the technique of lentivirus transfection.The cells with normal and high expression of TLR4 were induced by LPS for0 h,2 h and 4 h.Inflammatory cytokines TNF-α,IL-6 and IL-8 in the c...
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