【摘要】 目的探讨富含γ-氨基丁酸（γ-aminobutyric acid,GABA）的米糠提取物（rice bran extracts,RBE）对Aβ_25-35致PC12细胞损伤的影响。方法 （1）将细胞分为对照组和不同浓度RBE处理组,RBE的浓度分别为50、100、200、400、800、1600和3200（μg/ml）,用CCK-8法检测RBE对PC12细胞活性的影响。（2）将细胞分为对照组（不加任何处理因素）、Aβ_25-35处理组（20μmol/L）、不同浓度RBE干预+Aβ_25-35处理组,RBE浓度分别为100、200、400、500、800、1000和1600（μg/ml）。RBE干预采用预孵育和共孵育2种方式,用CCK-8法检测RBE对PC12细胞的保护作用;并比较2种不同孵育方式的保护效果。结果 （1）一定浓度范围内的RBE可提高PC12细胞的活性。（2）RBE预孵育的浓度为200¹000μg/ml时,对Aβ_25-35损伤的PC12细胞具有显著保护作用（P<0.05）,其最佳浓度为800μg/ml;RBE共孵育的浓度为400¹000μg/ml时,对Aβ_25-35损伤的PC12细胞具有显著保护作用（P<0.01）,其最佳浓度为1000μg/ml。结论适宜浓度的米糠提取物干预对Aβ_25-35损伤的PC12细胞具有保护作用。更多还原

【Abstract】 Objective To investigate the neuroprotective effects of rice bran extracts（RBE） rich in γ-aminobutyric acid（GABA） on PC12 cells. Methods 1. PC12 cells were divided into control group and RBE treated groups（at the concentrations of 50, 100, 200, 400, 800, 1600, 3200 μg/ml, respectively）. Cell viability was determined by CCK-8 assay. 2. PC12 cells were divided into control group, Aβ_25-35 treated group and RBE+Aβ_25-35 treated groups（RBE at the concentrations of 100, 200, 400, 500, 800, 1600 μg/ml, respectively）. The cells was pretreated with RBE before exposed to Aβ_25-35 or co-treated with RBE and Aβ_25-35. Cell viability was determined by CCK-8 assay. Results（1） Suitable concentrations of RBE increased cell viability of PC12 cells.（2） RBE pre-treatment at the concentrations of 200-1000 mg/ml had significant protective effects on PC12 cells exposed to Aβ_25-35（P<0.05）, and 800 mg/ml was considered the most suitable concentration. RBE co-incubation at the concentrations of 400-1000 mg/ml had significant protective effects on PC12 cells exposed to Aβ_25-35（P<0.01）, and 1000 mg/ml was considered the most suitable concentration. Conclusion Suitable concentrations of rice bran extracts have protective effects against injury induced by Aβ_25-35 in PC12 cells.更多还原