【摘要】 目的探讨幽门螺旋杆菌(Hp)感染与胃癌组织中细胞周期调节因子p27、细胞周期蛋白D1(CyclinD1)、巨噬细胞移动抑制因子(MIF)蛋白表达的相关性。方法收集2012年3月至2017年3月经胃镜与病理检查明确诊断的胃癌标本60例,根据是否发生Hp感染分为Hp阳性组、Hp阴性组各30例,另以正常胃黏膜组织20例为正常黏膜组,采用免疫组织化学法检测三组p27、CyclinD1、MIF蛋白表达水平,并分析其与胃癌患者临床病理特征及与Hp感染的相关性。结果 Hp阳性组p27阳性率低于Hp阴性组和正常黏膜组,CyclinD1、MIF蛋白表达水平高于阴性组和正常黏膜组(P<0.05),Hp阴性组与正常黏膜组上述指标差异也有统计学意义(P<0.05);p27、CyclinD1、MIF表达水平与临床分期、肿瘤浸润深度、肿瘤分化程度有关(P<0.05);胃癌患者Hp感染与p27表达水平呈负相关,与CyclinD1、MIF表达水平呈正相关,且p27与CyclinD1、MIF水平呈负相关性(P<0.05)。结论幽门螺旋杆菌感染与胃癌组织中p27、CyclinD1、MIF蛋白表达水平存在一定相关性,p27、CyclinD1、MIF可能参与胃癌发生发展。更多还原

【Abstract】 Objective To analyze the correlation between Helicobacter pylori (Hp) infection and the protein expressions of cell cycle regulatory factor p27,cyclinD1 and macrophage migration inhibitory factor (MIF) in gastric cancer.Methods Sixty gastric cancer specimens confirmed by gastroscopy and pathology were collected between March 2012 and March 2017.According to the occurrence of Hp infection,they were divided into Hp positive or negative groups.Another 20 normal gastric mucosa tissues were collected as normal mucosa group.The expression levels of p27,cyclinD1 and MIF in the three groups were detected by immunohistochemistry,and their correlations with the clinicopathological features and Hp infection were analyzed.Results The positive rate of p27 in the Hp positive group was lower than that in the Hp negative group and the normal mucosa group while the expression levels of cyclinD1 and MIF in the Hp positive group were higher than those in the Hp negative group and the normal mucosa group (P < 0.05).There were significant differences in above indexes between the Hp negative group and the normal mucosa group (P < 0.05).The expression levels of p27,cyclinD1 and MIF were related to clinical stage,depth of tumor invasion and tumor differentiation degree (P < 0.05).Correlation analysis also showed that Hp infection was negatively correlated with the expression level of p27,and positively correlated with the expression levels of cyclinD1 and MIF.There was a negative correlation between p27 and cyclinD1 and MIF levels (P < 0.05).Conclusion There is correlation between Hp infection and the expression levels of p27,cyclinD1 and MIF proteins in gastric cancer tissues.P27,cyclinD1 and MIF may be involved in the development of gastric cancer.更多还原