【摘要】 目的观察药根碱对糖尿病大鼠血管蛋白激酶B/一磷酸腺苷活化蛋白激酶/内皮型一氧化氮合酶（Akt/AMPK/e NOS）信号通路的影响及对糖尿病大鼠可能的保护作用机制。方法将60只雄性Wistar大鼠随机分为正常对照组、模型对照组、药根碱小剂量组和大剂量组,除正常对照组外,其他组通过诱导胰岛素抵抗后空腹腹腔注射链脲佐菌素制作2型糖尿病模型,正常对照组和模型对照组大鼠每日灌胃5%羧甲基纤维素钠溶液,药根碱大剂量组和小剂量组每日分别给予药根碱100,50 mg·kg^-1,连续8周。检测各组大鼠体质量、血糖及血清胰岛素水平,酶联免疫吸附测定（ELISA）法比较各组大鼠血清中炎症因子白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）水平,Western blotting法检测各组大鼠血管中e NOS蛋白及Akt/AMPK通路蛋白的表达。结果与正常对照组比较,模型对照组大鼠体质量下降,血糖升高,血清胰岛素水平显著性升高,差异有统计学意义（P<0.01）;与模型对照组比较,药根碱大剂量组体质量显著增加,血糖降低,胰岛素水平下降均差异有统计学意义（P<0.01）。正常对照组血清中IL-1β为（92.3±4.3）pg·mL^-1,模型对照组为（152.4±20.0）pg·mL^-1,药根碱小剂量组和大剂量组分别为（120.96±33.0）,（95.05±7.7）pg·mL^-1;模型对照组血清中TNF-α为（10.50±0.82）pg·mL^-1,正常对照组为（7.48±0.36）pg·mL^-1,药根碱小剂量组和大剂量组TNF-α分别为（8.82±0.42）和（7.11±0.33）pg·mL^-1;与正常对照组比较,模型对照组大鼠血管中e NOS表达量及磷酸化的Akt和AMPK含量显著减少,药根碱大剂量组较模型对照组显著提高（P<0.05或P<0.01）。结论药根碱可能通过影响糖尿病大鼠血管中Akt/AMPK/e NOS信号通路及对抗炎症作用对糖尿病大鼠具有保护作用。更多还原

【Abstract】 Objective To observe the influence of jatrorrhizine on the Akt/AMPK/e NOS signaling pathways and potential protective function in blood vessel of diabetes rats. Methods Male Wistar rats（ n = 60） were randomly divided into normal control group,model control group,low-and high dose jatrorrhizine groups. Except normal control group,the other rats were given intraperitoneal injection of STZ after induced insulin resistance,to made type Ⅱdiabetes model. CMC-Na solution（ 5%） was given to normal control and model control group, and the jatrorrhizine resolved in the same solution was administered to low( 50 mg·kg^-1) and high dose( 100 mg·kg^-1) jatrorrhizine groups for 8 weeks. Their body weight,blood glucose,and serum insulin levels were measured at the end of the treatment,IL-1β,TNF-α level in serum were measured by ELISA,and the e NOS,Akt/AMPK protein expression levels in the blood vessel were measured by Western blotting. Results Compared with normal control group,the weight of model control group was lossed,blood glucose was increased（ P<0.01）. Compared with model control group,high-dose jatrorrhizine significantly increased body weight,alleviated blood glucose and decreased serum insulin（ P<0.01）.Serum inflammatory factor like IL-1β was（ 92.3±4.3） pg·mL^-1 in normal control group,（ 152.4±20.0） pg·mL^-1 in model control group,（ 120.96±33.0） pg·mL^-1 and（ 95.05±7.7） pg·mL^-1 in low-and high-dose jatrorrhizine groups,respectively. TNF-α was（ 10.50±0.82） pg·mL^-1 in model control group,（ 7.48±0.36） pg·mL^-1 in normal control group,（ 8.82±0.42） and（ 7.11±0.33）pg· mL^-1 in low-and high-dose jatrorrhizine groups,respectively. As compared with control group,e NOS and Akt/AMPK expression in blood vessel was significantly reduced（ P < 0. 05） in model control group,and those were significantly increased in high-dose jatrorrhizine group as compared with model control group（ P<0.05 or P<0.01）. Conclusion Jatrorrhizine may exert protective effect on diabetes mellitus rats through regulating Akt/AMPK/e NOS signaling pathway in blood vessel.更多还原