【摘要】 目的建立钩菊胶囊的质量标准。方法薄层色谱法对钩藤、淫羊藿进行定性鉴别;UV-HPLC对淫羊藿苷进行含量测定。采用Agilent ZORBAX SB-C₁₈色谱柱（150 mm×4.6 mm,5μm）;流动相:乙腈-水（25∶75）;流速:1.0 mL/min;柱温:30℃;检测波长为270 nm。结果薄层色谱中斑点清晰,阴性均无干扰;淫羊藿苷在15¹50μg/mL范围内线性关系良好（r=0.9996）,平均回收率为100.9%（RSD=2.77%）。结论本试验结果准确、简便,易控,可作为钩菊胶囊的质量标准。更多还原

【Abstract】 Objective To establish the quality standard of Gouju Capsules. Methods The main ingredients of Ramudus Uncaria Cum Uncis, Epimedium were identified by TLC. Content of icariin was determined by UV-HPLC. The content of the berberine was determined by HPLC; the column of Agilent ZORBAX SB-C₁₈（150 mm×4.6 mm, 5 μm） was used.The mobile phase was ocetonitrile-water（25∶75）, the column temperature of 30℃, at the flow of 1.0 mL/min and detec tion wavelength was 270 nm. Results The spots on TLC plates were distinct, without interference. A good linearity of icariin was in the ranges of 15-150 μg/mL（r=0.9996）. The average recovery was 100.9%（RSD =2.77%）. Conclusion The results of this experiment are accurate, simple and easy to control. It can be used as a quality standard for Gouju Capsules.更多还原