【摘要】 目的建立Balb/c裸小鼠寻常型银屑病动物模型,观察银屑平对其表皮组织中双调蛋白(AREG)、血管内皮生长因子(VEGF)及角质形成细胞生长因子(KGF)的影响。方法 Balb/c裸小鼠36只,随机分为模型对照组和银屑平A组、银屑平B组,三组均连续8 d于小鼠头颈部、上肢、躯干和下肢4个部位皮肤涂咪喹莫特软膏的方法建模。末次给药造模第8天,银屑平A、B组按0.1 g/kg和0.5 g/kg剂量灌胃治疗10 d,模型对照组灌等量生理盐水。于造模第8天和治疗第10天,采用皮损面积和严重程度指数(PASI)对动物头颈部、上肢、躯干和下肢4个部位皮肤病变情况进行评分;ELISA法定量测定表皮组织匀浆液中AREG、KGF、VEGF和血管内皮生长因子受体1(VEGFR1)等指标含量。结果银屑平A组、银屑平B组和模型对照组造模第8天PASI评分比较,差异无统计学意义(P>0.05),灌胃治疗第10天时,银屑平A组、银屑平B组PASI评分均较模型对照组和同组造模第8天明显降低(P<0.05),表皮组织匀浆液中AREG、KGF、VEGF和VEGFR1均低表达(P<0.05)。银屑平A组、银屑平B组组间比较,差异无统计学意义(P>0.05)。结论银屑平可能通过降低表皮组织匀浆液中AREG、KGF、VEGF含量,并有可能阻断VEGF与和VEGFR1结合,这对抑制表皮病变细胞角化和皮下组织血管新生,减轻受试动物银屑病样病理改变有肯定的干预作用。更多还原

【Abstract】 Objective To establish animal models of Balb/c nude mice with psoriasis vulgaris, observe the effect of Yinxieping on double adjustable epidermal tissue protein(AREG), vascular endothelial growth factor(VEGF) and keratinocyte growth factor(KGF). Methods 36 Balb/c nude mice were randomly divided into model control group and Yinxieping A group, Yinxieping B group, the three groups were all smeared imiquimod cream for consecutive 8 d in skin of4 sections in head and neck, upper limbs, torso and lower extremities of mice to built the model. At the dose of 8th day after modeling, Yinxieping A group, Yinxieping B group were treated by gavage for 10 d with 0.1 g/kg and 0.5 g/kg dose, model group was injected into the same amounts saline. At the treatments 8th, 10 th day, psoriasis area and severity index(PASI) was used to evaluated the animal head neck, upper limbs, torso and lower extremities 4 skin lesions;ELISA was uesd to determine contents of AREG, KGF, VEGF and vascular endothelial growth factor receptor 1(VEGFR1) and other indicators in epidermal tissue homogenates in a fixed quantity. Results PASI scores of Yinxieping A group, Yinxieping B group and model control group at modeled 8th day were compared, the differences were not statistically significant(P > 0.05), when treated by gavage at10 th day, compared with the model control group and themsavies at modeled 8th day, PASI scores of Yinxieping A group, Yinxieping B group decreased significantly(P < 0.05), the expression of AREG, KGF, VEGF and VEGFR1 in epidermal tissue homogenates was lower(P > 0.05). Yinxieping A group and Yinxieping B group were compared, the differences were not statistically significant(P > 0.05). Conclusion Yinxieping can decrease contents of AREG, KGF, VEGF in epidermal tissue homogenate, and it may block the combination of VEGF and VEGFR1, which has positive effects in the inhibition of epidermal keratinocyte lesions and subcutaneous tissue angiogenesis, reducing the subjects change in animal with psoriasis to play parts in pathological intervention.更多还原