【摘要】 目的研究外周给精氨酸加压素（AVP）对脂多糖（LPS）引起大鼠发热和棕色脂肪产热以及血清中IL-1β和PGE2水平的变化。方法实验用成年雄性SD大鼠,在23℃环境温度下,明暗时间各12h。用无线遥测系统连续测量大鼠体核温度（Tc）、棕色脂肪温度(T_BAT)和活动。10:00时或11:30时分别给大鼠腹腔注射LPS（50μg/kg）、AVP（10μg/kg）或V1a受体阻断剂（30μg/kg）。用ELISA法测定血清IL-1β和PGE₂的含量。结果腹腔注射LPS后TBAT升高出现在Tc升高之前。腹腔V1a受体阻断剂能提高LPS引起发热反应。AVP能抑制LPS引起发热大鼠血清中IL-lβ和PGE2水平升高反应。结论 BAT产热在LPS引起的发热中有重要的作用。外周给AVP可通过抑制棕色脂肪产热和降低血液中IL-lβ和PGE2的浓度而翻转LPS发热反应。内源性AVP也有限制LPS发热的作用。更多还原

【Abstract】 Objective To investigate the effect of peripheral administration of arginine vasopressin（ AVP） on lipopolysaccharide（ LPS）-induced fever in the rats,and its relationship with the change in brown adipose tissue（ BAT） thermogenesis and Interleukine-1β（ IL-1β） and prostaglandin E₂（ PGE₂）. Methods The core temperature（ Tc）,BAT temperature( T_BAT) and activity were measured by telemetry in adult male Sprague-Dawley rats at an ambient temperature of 23℃ during a 12 h light∶ 12 h dark photoperiod（ lights on at 06: 00 and lights off at 18: 00）. The rats were dosed intraperitoneally with LPS（ 50μg/kg）,AVP（ 10μg/kg） or V1 a vasopressin receptor antagonist（ V1 a antagonist,30μg/kg） at 10: 00 or 10: 30. The concentrations of IL-1β and PGE2 in serum were tested by ELISA. Results（ 1） The increases in TBATfollowing LPS preceded the increases in Tc.（ 2） Peripheral administration of AVP reversed LPS-induced fever and decreased BAT thermogenesis.（ 3） V1 a antagonist enhanced the fever produced by LPS.（ 4） AVP could significantly attenuate LPS-induced IL-1β and PGE₂ production. Conclusion（ 1） BAT thermogenesis plays an important role in LPS-induced fever.（ 2） Peripheral administration of AVP reversed LPS-induced fever by reduction of BAT thermogenesis and inhibition of IL-1β and PGE₂.（ 3） Endogenous AVP can attenuate fever induced by LPS.更多还原