【摘要】 目的 探讨妊娠晚期乙二胺四乙酸依赖性假性血小板减少(EDTA-PTCP)的发生机制及准确诊断与准确计数血小板方法,为临床提供参考。方法 报告2例妊娠晚期EDTA-PTCP产妇分娩前后及新生儿血小板值,并追溯其第一次妊娠分娩前后血小板值。结果 1例分娩前EDTA抗凝管查PLT 8×10~9/L,可见血小板聚集,重新采血后不加抗凝剂行人工计数PLT 189×10~9/L;新生儿第3天EDTA抗凝管查PLT 291×10~9/L。另1例分娩前EDTA抗凝管查PLT 7×10~9/L,可见血小板聚集,重新采血后不加抗凝剂行人工计数PLT 137×10~9/L;新生儿第3天EDTA抗凝管查PLT 248×10~9/L。结论 利用血液学分析仪分析血小板和白细胞典型柱状图可准确诊断EDTA-PTCP。在EDTA血液样品试管中加入氨基糖苷类、镁盐或者柠檬酸盐、5-磷酸吡哆醛和3-磷酸吡哆醛,可以准确计数血小板;或在血液样品中加入氨基糖苷类,通过1.25倍稀释因子分析红细胞沉降率,也能获得准确的PLT计数。更多还原

【Abstract】 Objective To investigate pathogenetic mechanism and accurate methods for diagnosing EDTA-PTCP and platelet counting in late trimester of pregnancy in order to provide a guidance for chnical diagnosis and treatment.Methods Platelet counts of two patients with EDTA-PTCP in late trimester of pregnancy before and after delivery as well as newborn were reported.Platelet counts were retrospected before and after delivery in the first pregnancy.Results PLT in EDTA anticoagulant tube for one case before delivery was 8 × 10~9 g/L and platelet aggregation was seen.PLT without anticoagulant was 189 × 10~9 g/L.PLT in EDTA anticoagulant tube for newborn at the third day was 291 × 10~9 g/L.PLT in EDTA anticoagulant tube for another case before delivery was 7 × 10~9 g/L and platelet aggregation was seen.PLT without anticoagulant was 137 × 10~9 g/L.PLT in EDTA anticoagulant tube for newborn at the third day was 248 × 10~9 g/L.Conclusion EDTA-PTCP can be diagnosed accurately by analyzing platelet and white blood cell histogram using hematology analyzer.PLT can be counted accurately by adding aminoglycosides,magnesium salts or citrates,5-phosphate pyridoxal and 3-phosphate pyridoxal to blood samples in EDTA tube.Analysis of erythrocyte sedimentation rates by 1.25 fold dilution factors by adding aminoglycosides to blood samples can also get accurate PLT counts.更多还原