【摘要】 目的探讨米胚粉饮食干预对Ⅱ型糖尿病模型SD雄性大鼠的影响,为防治Ⅱ型糖尿病的相关并发症提供科学依据。方法 102只SD雄性大鼠随机分为Ⅱ型糖尿病模型组和正常对照组,前者给予高脂高糖饲料和小剂量STZ后,再随机分为模型组、阳性对照组及米胚粉低、中、高剂量组,分别给予普通饲料、普通饲料+500mg/kg二甲双胍溶液灌胃、2.5%、10%、40%米胚粉饲料,正常对照组给予普通饲料。饮食干预4周后,计算各组大鼠死亡率,并检测血清及肝脏抗氧化指标(SOD、MDA、GSH-PX)和血脂指标(TG、CHO、LDL-C)。结果高剂量组无死亡发生,高剂量组与模型组相比较,血清与肝脏中SOD显著增加[分别为(44.81±6.17)、(150.36±24.11)U/ml](P<0.05),MDA显著降低[分别为(7.53±1.84)、(7.07±1.13)U/ml](P<0.05),且血清CHO与LDL-C明显降低[分别为(2.57±0.41)、(1.04±0.47)mmol/L](P<0.05)。结论米胚粉对Ⅱ型糖尿病大鼠具有降血脂、改善氧化损伤、降低死亡率的作用。更多还原

【Abstract】 Objective To explore the effect of rice germ diet intervention on an SD male rats model of type Ⅱdiabetes,so as to provide the scientific basis for prevention and cure of related complications of type Ⅱdiabetes. Methods A total of 102 male SD rats were randomly divided into two groups:the type Ⅱdiabetes model group and the normal control group.The model group were fed with high fat diet and injected with a single dose of STZ,and then were randomly divided into five groups,namely the model group,the positive control group and rice germ of low,middle and high dose group,and were fed with normal diet and normal diet plus metformin 500 mg/kg solution,2.5% ,10% and 40% rice germ respectively.Normal control group were fed with normal diet.After 4 weeks of dietary intervention,the mortality of rats in each group was calculated.Serum and liver tissue were collected to measure the oxidant-antioxidant status(SOD,MDA,GSHPX).Serum TG,CHO and LDL-C were also determined. Results No rat death occurred in the high dose group,as compared to the model group,the activity of SOD in serum and liver tissue in the high dose group significantly increased(44.81±6.17 U/ml,150.36±24.11 U/ml)(P <0.05),whereas the level of MDA significantly decreased(7.53±1.84 U/ml,7.07±1.13 U/ml)(P <0.05)and the levels of serum CHO and LDL-C also significantly decreased(2.57±0.41 mmol/L,1.04±0.47 mmol/L)(P <0.05). Conclusion Rice germ can lower blood lipid,improve oxidative damage,and reduce mortality of type Ⅱdiabetic rats.更多还原