【摘要】 目的探讨益心脑滴丸对肾性高血压大鼠（RHR）的降压作用及机制。方法构建两肾一夹（2K1C）经典肾性高血压大鼠模型,选取造模成功的大鼠并按收缩压水平分为模型组,益心脑高、中、低剂量组(2,1,0.5 g·kg^-1·d^-1)及卡托普利组(25 mg·kg^-1·d^-1),同时设立假手术组,每组8只。灌胃给药每日1次,每周末测量血压,6周后处死并检测血浆内皮素（ET）和一氧化氮（NO）,总抗氧化能力（T-AOC）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和肾组织活性氧（ROS）、谷胱甘肽过氧化物酶（GSH-Px）的变化;用酶联免疫法（ELISA）检测血浆肾素活性（RA）及血管紧张素Ⅰ（AngⅠ）,AngⅡ的含量。结果益心脑滴丸能明显降低高血压大鼠收缩压（SBP）（P<0.05）;增加血浆NO,降低ET含量（P<0.05）;降低ROS和MDA的含量,提高抗氧化酶SOD,CAT和GSH-Px活性（P<0.05）,使T-AOC增强;升高血浆AngⅠ含量,降低血浆AngⅡ含量（P<0.05）。结论益心脑滴丸有一定的降压作用,其机制可能与抗氧化,影响肾素-血管紧张素系统有关。更多还原

【Abstract】 Objective To investigate the antihypertensive effect of Yixinnao dripping pills on renal hypertensive rats（RHR） and its mechanism. Methods The two-kidney one-clip（2K1C） goldblatt RHR models were established. The successfully established rat models were randomly divided into the model group, high, medium and low dose Yinxinnao groups(2, 1, 0.5 g·kg^-1·d^-1) and captopril group(25 mg·kg^-1·d^-1), and the sham operation group was established（n=8 each）. All the rats received intragastric administration once daily and the blood pressure determina-tion every weekend, and were sacrificed at 6 weeks. The changes of plasma endothelin（ET）, nitric oxide（NO）, total anti-oxidation capability（T-AOC）, malondialdehyde（MDA）, superoxide dismutase（SOD）, catalase（CAT）, reactive oxy-gen species（ROS） of renal tissues, and glutathione peroxidase（GSH-Px） were determined. The levels of plasma renin activity（RA）, angiotensin Ⅰ, Ⅱ（Ang Ⅰ, Ang Ⅱ） were measured by enzyme-linked immunosorbent assay（ELISA）.Results Yixinnao dripping pills could significantly decrease the systolic blood pressure（SBP） of hypertensive rats（P<0.05）, increase the plasma NO and decrease the ET level（P<0.05）. T-AOC could be enhanced by decreasing the levels of ROS and MDA, and increasing the activities of antioxidant enzymes SOD, CAT and GSH-Px（P<0.05）. The level of plasma Ang I was increased, and the plasma Ang Ⅱ was decreased（P<0.05）. Conclusion Yinxinnao dripping pills may have a certain antihypertensive effect, and its mechanism may be related to antioxidant, affecting the renin-angiotensin system.更多还原