【摘要】 目的观察斑点型锌指结构蛋白(SPOP)在膀胱癌组织及细胞株中表达情况。方法采用实时定量逆转录聚合酶链反应(qRT-PCR)和蛋白质印迹法分别检测膀胱癌组织与正常膀胱黏膜组织,不同膀胱癌细胞株与正常膀胱黏膜上皮细胞株SPOP mRNA表达量和SPOP蛋白表达量,比较分析两者表达情况。结果膀胱癌细胞株5637,T24,J82及正常膀胱细胞株SV-HUC-1中SPOP mRNA表达量分别为1.02±0.78,1.12±0.49,1.07±0.28,0.96±0.28;SPOP蛋白表达水平分别为0.44±0.16、0.38±0.27、0.14±0.33,0.72±0.24,膀胱癌细胞株与正常膀胱细胞株在SPOP mRNA水平表达差异无统计学意义(P>0.05)。而SPOP蛋白表达水平在膀胱癌细胞株与正常膀胱细胞株间差异有统计学意义(P<0.05)。SPOP mRNA表达水平在膀胱癌组织与正常膀胱黏膜组织间表达量差异无统计学意义(P>0.05),而SPOP蛋白表达水平在膀胱癌组织与正常膀胱黏膜组织间表达差异有统计学意义(P<0.05)。结论 SPOP在膀胱癌组织中表达降低,在膀胱癌发生发展中可能起到抑制作用。SPOP可能通过转录后机制调控膀胱癌进程。更多还原

【Abstract】 Objective To determine the expression of SPOP in bladder cancer tissues and cell lines. Methods Real-time quantitative reverse transcriptase polymerase chain reaction(qRT-PCR) and Western blotting were used to examine the expression of bladder cancer tissues and normal bladder mucosa tissues. The SPOP mRNA and protein expression in different bladder cancer cell lines and normal bladder mucosa epithelial cell lines were compared and analyzed. Results The expression levels of SPOP mRNA in bladder cancer cell lines 5637, T24, J82 and normal bladder cell line SV-HUC-1 were 1.02±0.78, 1.12±0.49, 1.07±0.28 and 0.96±0.28, respectively. The expression levels of SPOP protein were 0.44±0.16, 0.38±0.27, 0.14±0.33 and 0.72±0.24, respectively. There was no statistically significant difference in the SPOP mRNA expression level between the bladder cancer cell lines and normal bladder cell lines(P>0.05). There was no significant difference in the expression level of SPOP mRNA between the bladder cancer tissues and normal bladder mucosa tissues(P>0.05), whereas there was significant difference in the expression level of SPOP protein between the bladder cancer tissues and normal bladder mucosa tissues( P <0.05). Conclusion The SPOP expression decreases in the bladder cancer tissues, which may inhibit the occurrence and development of blad-der cancer. SPOP may regulate the process of bladder cancer through post-transcriptional mechanism.更多还原