【摘要】 目的:建立酰胺基亲水作用色谱-高效液相色谱-紫外法测定DHA颗粒中磷脂酰丝氨酸的含量。方法:以正己烷-异丙醇为提取溶剂对样品进行超声提取;选用Waters XBridge Amide（4.6 mm×250 mm,3.5μm）色谱柱,以乙腈-异丙醇（7∶3）为流动相,流速1.0 m L·min^-1,柱温45℃,进样量20μL,紫外检测波长203 nm,单次运行时间10 min。结果:样品中磷脂酰丝氨酸获得很好的分离,在0.1¹.0mg·m L^-1线性关系良好（r=0.999 9）;在高、低浓度的回收率（n=6）分别为101.2%和95.8%,RSD分别为1.3%和2.6%;3批样品中磷脂酰丝氨酸含量分别为10.5、10.3、10.6 mg·g^-1。结论:本方法操作简捷、环保、高效,经方法学验证,本法专属性、重复性好,准确度高,适应性强,为含磷脂酰丝氨酸功能食品的全面质量控制提供一种新的方法。更多还原

【Abstract】 Objective:To establish an Amide-HPLC-UV method for the determination of phosphatidylserine in DHA granules.Methods:The sample was extracted with n-hexane-isopropanol as the extraction solvent.The chromatographic separation was achieved on a Waters XBridge Amide（4.6 mm×250 mm,3.5μm）column using acetonitrile-isopropanol（7∶3）as the mobile phase at a flow rate of 1.0 m L·min^-1.The column temperature was45℃,the injection volume was 20μL,the detection wavelength was set at 203 nm,and the single run time was 10min.Results:Phosphatidylserine in the sample was well separated.The calibration curves of phosphatidylserine showed good linearity in the range of 0.1-1.0 mg·m L^-1（r=0.999 9）.The average recoveries（n=6）at high and low concentrations were 101.2%and 95.8%,respectively;and RSD were 1.3%and 2.6%,respectively.The contents of phosphatidylserine in three batches were 10.5,10.3,10.6 mg·g^-1,respectively.Conclusion:This method is simple,environmental and efficient.The established method is proved by methodology validation which is specific,reproducible,accurate and adaptable,that can be applied to overall quality control of phosphatidylserine in functional food.更多还原