【摘要】 目的:研究老年骨质疏松患者外周血清成纤维生长因子(fibroblast growth factor,FGF)及Klotho的表达。方法:根据骨影像学检查结果,选取46例老年骨质疏松患者(O组)及30例健康者(C组)纳入本研究。采用酶联免疫吸附反应(ELISA)分别检测血清中FGF21、FGF23、α-Klotho及β-Klotho的表达水平,并与双能X射线骨密度检测t值进行相关性分析。结果:O组患者血清FGF21的表达较C组差异无统计学意义(P>0.05),但呈现轻度下降趋势;与C组相比,O组患者外周血清FGF23表达显著升高,且血清α-Klotho及β-Klotho的表达水平均显著下调,差异有统计学意义(P<0.05);再者,Pearson相关性分析提示骨密度t值与血清FGF23的表达显著负相关,而与血清α-Klotho及β-Klotho的水平显著正相关,差异有统计学意义(P<0.05)。结论:血清FGF23、α-Kloth及β-Klotho的表达异常可能与骨质疏松的发病机理有关,且其可用于临床辅助诊断骨质疏松。更多还原

【Abstract】 Objective:To determine the serum levels of fibroblast growth fctor and Klotho of aged patients with osteoporosis.Method:On the basis of the clinical results of imageological examination,46 aged osteoporosis patients(O group) and 30 healthy individuals(C group) were enrolled in the present study.Enzyme-linked immunosorbent assay(ELISA) was respectively applied to detect the serum levels of FGF21、FGF23、α-Klotho and β-Klotho. Moreover,statistical analysis of Pearson correlation was used to analyze the correlationship between T value of dual energy X-ray absorptionmetry and FGF, as well as Klotho.Result:Serum level of FGF21 in O group showed no significantly statistical difference compared with C group(P>0.05),while it displayed a slighter decrease.Compared with C group,patients in O group demonstrated a significant increase of FGF23,and that serum levels of α-Klotho and β-Klotho both significantly down-regulated(P<0.05);Furthermore,Pearson analysis suggested that T value of bone mineral density was negatively correlated with serum FGF23 level,and negatively correlated with both serum levels of α-Klotho and β-Klotho(P<0.05).Conclusion:Abnormal serum FGF23,α-Kloth and β-Klotho levels are both highly associated with the pathogenesis of osteoporosis,which could be used as promising biomarkers for auxiliary diagnosis of osteoporosis.更多还原