【摘要】 目的探讨Ki67在非小细胞肺癌(non-small cell lung carcinoma,NSCLC)组织中的表达及其与多药耐药基因谷胱甘肽-S转移酶π(GST-π)、拓扑异构酶Ⅱ(TopoⅡ)、P-糖蛋白(P-gp)表达的相关性及其意义。方法采用免疫组化法检测108例NSCLC患者癌组织中Ki67、GST-π、TopoⅡ、P-gp的表达水平。结果 Ki67、GST-π、TopoⅡ、P-gp在108例NSCLC患者癌组织中的阳性表达率分别为95.37%、81.48%、82.40%、38.89%;GST-π表达与NSCLC分化程度有关(χ~2=6.318,P=0.042);Ki67表达与TopoⅡ表达有关(χ~2=6.503,P=0.011),共表达率为80.56%,但关系不密切(r=0.245,P=0.010);Ki67表达与GST-π表达有关(χ~2=4.686,P=0.03),共表达率为76.85%,但是相关性低(r=0.028,P=0.031);Ki67表达与P-gp表达无关(P>0.05)。GST-π与TopoⅡ表达呈正相关(r=0.028,P=0.023),共表达率为70.37%,但是关系不密切;P-gp与GST-π、TopoⅡ无相关(P>0.05)。结论 Ki67可能不参与NSCLC细胞的多药耐药机制;三种多药耐药基因可能是通过不同的途径介导NSCLC多药耐药。更多还原

【Abstract】 Objective To investigate the expression of Ki67 in non-small cell lung cancer (NSCLC) and its correlation with expressions of multidrug resistance genes glutathione-S-transferase π (GST-π),topoisomeraseⅡ (TopoⅡ) and P-glycoprotein (P-gp) and significance.Methods The expression levels of Ki67,GST-π,TopoⅡand P-gp in tissues of 108 NSCLC cases were detected by immunohistochemistry.Results The positive expression rates of Ki67,GST-π,TopoⅡ and P-gp in tissues of 108 patients with NSCLC were 95.37%,81.48%,82.40% and 38.89%,respectively.The expression of GST-π was associated with the degree of NSCLC differentiation (χ~2=6.318,P =0.042);Ki67 expression was associated with TopoⅡexpression (χ~2=6.503,P =0.011),the co-expression rate was 80.56%,but the correlation was not significant (r=0.245,P=0.010);Ki67 expression was associated with GST-πexpression (χ~2=4.686,P =0.03),the co-expression rate was 76.85%,but the correlation was low (r=0.028,P =0.031);Ki67 expression was not associated with P-gp expression(P >0.05).GST-πwas positively correlated with TopoⅡ expression(r=0.028,P =0.023),and the co-expression rate was 70.37%,but the relationship was not close;P-gp was not associated with GST-πand TopoⅡ (P >0.05).Conclusion Ki67 may not participate in the multidrug resistance mechanism of NSCLC cells;three multidrug resistance genes may mediate NSCLC multidrug resistance by different ways.更多还原