【摘要】 目的对实时荧光定量逆转录-聚合酶链反应(rRT-PCR)法和微量中和实验2种脊灰病毒的血清型鉴定方法进行评价。方法按世界卫生组织(WHO)《脊髓灰质炎实验室手册》第4版进行病毒分离,用rRT-PCR法和微量中和实验方法对分离到的L20B阳性分离物进行PV的血清型鉴定,用rRT-PCR法进行型内鉴定。结果 rRT-PCR法与微量中和实验对L20B阳性分离物进行脊灰病毒的血清型定型,2种方法检测脊灰病毒阳性率差异无统计学意义,检测NPEV阳性率差异也无统计学意义;rRT-PCR方法检测脊灰病毒的灵敏度为100%,特异度为100%;检测NPEV的灵敏度为63.16%,特异度为100%。结论作为WHO推荐使用的新的型内鉴定方法,rRT-PCR法对于脊灰病毒的血清型定型与常规微量中和实验一致率为100%,该方法方便快捷,可以缩短检测时限;对于NPEV的检测特异度较高,但灵敏度低于微量中和实验。更多还原

【Abstract】 Objective To evaluate real-time reverse transcription-polymerase chain reaction(rRT-PCR)method and micro-neutralization assay for serotyping of polivirus.Methods Isolation of virus was performed according to the World Health Organization(WHO)Poliomyelitis Laboratory Manual(the 4th edition),PV serotyping was performed for L20 Bpositive isolates by rRT-PCR and microneutralization assay,and intratypic differentiation by rRT-PCR.Results No statistically significant difference was found between polivirus positive rates or NPEV positive rates obtained by the two methods.rRT-PCR was 100% sensitive and 100% specific to polivirus;and 63.16% sensitive and 100% specific to NPEV.Conclusion As a new method for intratypic differentiation recommended by WHO,rRT-PCR yields the same results as routine micro-neutralization method for serotyping of polivirus.The method is simple and convenient and can shorten the test duration;it is highly specific to NPEV but not as sensitive as micro-neutralization method.更多还原