【摘要】 目的 观察纳米羟基磷灰石/细菌纤维素(nHA/BC)复合支架对兔骨髓间充质干细胞(BMSCs)的黏附及成骨分化的影响，以探讨其在骨组织工程领域潜在的应用价值。方法 培养及鉴定兔BMSCs,将BMSCs与nHA/BC体外复合，倒置相差显微镜及扫描电镜观察BMSCs与nHA/BC的复合情况。利用诱导培养基诱导细胞成骨分化，观察细胞分化过程中的形态变化、扫描电镜观察矿化基质沉积。结果 第3代BMSCs呈均匀一致的长梭形，流式细胞仪检测其表面抗体CD44阳性，CD45阴性。倒置相差显微镜下观察BMSCs能够在表面有效的黏附生长，保持细胞形态，至7天时细胞融合成片。扫描电镜观察：nHA/BC呈三维立体多孔结构，BMSCs与nHA/BC复合后，BMSCs伸出伪足黏附在表面及孔隙内，细胞相互连接、融合。细胞诱导后呈现明显的成骨细胞特征，可见钙结节生成。扫描电镜观察可见nHA/BC膜上明显的矿物质沉积，并与nHA/BC膜紧密相连，结节中可见大量粗大的颗粒。结论 nHA/BC能促进兔BMSCs黏附，为成骨分化提供结构支撑。更多还原

【Abstract】 Objective To observe the effects of nano-hydroxyapatite/bacterial cellulose(nHA/BC) on the adhesion and osteoblastic differentiation of bone marrow mesechymal stem cells(BMSCs), and explore the feasibility and potential applications of nHA/BC in the field of bone tissue engineering. Methods Rabbit BMSCs were isolated and identified before adhesion on nHA/BC for phase contrast microscopy and scanning electron microscopy(SEM). Then, BMSCs were induced for osteoblastic differentiation. The morphological changes of BMSCs and the deposition of mineralized matrix on nHA/BC were observed. Results BMSCs became long fusiform-shaped at the third generation. According to flow cytometry, they were CD44 positive and CD45 negative. Under the phase contrast microscope, BMSCs grew on the surface of nHA/BC with the same cell morphology until attachment of each cell on Day 7. The SEM images showed that nHA/BC was in a three-dimensional porous structure, while BMSCs attached to and fused with each other through adhesion on the surface and within the pores of nHA/BC through pseudopodia. After inducement, BMSCs showed obvious profiles of osteoblasts, and calcium nodules were found. According to SEM images, deposited minerals were clearly seen on the surface of nHA/BC membrane, and closely linked with the membrane, with a large number of coarse particles in the nodules. Conclusions nHA/BC can stimulate the adhesion of rabbit BMSCs, and provide structural evidence for osteogenic differentiation.更多还原