【摘要】 目的评估各染色方法在周围神经虚拟三维重建中应用可行性。方法采集临床胫神经标本,将处理好的标本切片分别用Karnovsky-Roots染色、乙酰胆碱酯酶染色、胆碱乙酰转移酶染色和碳酸酐酶染色4种不同染色,拍照观察,评估各染色方法。结果 Karnovsky-Roots和胆碱乙酰转移酶染色部位与背景区域视觉差异明显,而乙酰胆碱酯酶染色和碳酸酐酶染色部位与背景区域视觉差异不明显。Karnovsky-Roots染色步骤最为简单。结论 Karnovsky-Roots法较为适合应用于周围神经虚拟重建。更多还原

【Abstract】 Objective To explore the feasibility of different histochemical staining in threedimensional reconstruction of peripheral nerves. Methods The samples were collected from fresh adult common tibial nerve,which were serially horizontally sliced with the thickness of 6( m. All slices were randomly divided into four groups with different staining such as Karnovsky-Roots staining,acetylcholinesterase staining,acetyltransferase staining or carbonic anhydrase staining. The stain characteristics in various stain phrases were observed under optic-microscope. Results The character of nerve fibers could be distinguished clearly by Karnovsky-Roots,acetylcholinesterase,acetyltransferase and acetyltransferase staining. It took 50 to 52 hours to finish the staining by using Karnovsky-Roots staining. Carbonic anhydrase staining was the simplest method. Conclusion Among the four staining methods,Karnovsky-Roots staining is suitable for three-dimensional reconstruction of peripheral nerves.更多还原