【摘要】 目的了解接触低剂量电离辐射医学放射工作人员机体免疫相关细胞因子的变化情况。方法采用典型抽样法,选取广东省7家三级甲等医院244名医学放射工作人员为研究对象,其中诊断放射学组、核医学组、放射治疗组、介入放射学组分别有61、51、74和58人。另选工作中不接触电离辐射的51名行政人员为对照组。采用热释光剂量仪对接触组人员进行为期1年的个人剂量监测。采用酶联免疫吸附实验检测各组人员血清中γ干扰素(IFN-γ)、白细胞介素10(IL-10)和转化生长因子β1(TGF-β1)表达水平。结果医学放射工作人员的最大人均年有效剂量为0.41 mSv/a,低于职业接触限值20.00 mSv/a。核医学组工作人员人均年有效剂量分别高于诊断放射学组、放射治疗组和介入放射学组(P<0.01)。男性工作人员中,诊断放射学组、放射治疗组和介入放射学组人员血清IL-10表达水平均低于对照组(P<0.05);放射治疗组人员的IL-10表达水平分别低于核医学组和介入放射学组(P<0.05);放射治疗组人员的IFN-γ/IL-10比值分别高于诊断放射学组、核医学组、介入放射学组和对照组(P<0.05)。将医学放射工作人员按人均年有效剂量分为低、中、高3个剂量组(0.03~、0.06~和>0.15 mSv/a),该3组男性工作人员的IL-10表达水平均低于对照组男性工作人员(P<0.05)。结论长期低剂量电离辐射可能对男性工作人员IL-10介导的体液免疫产生一定的抑制作用。更多还原

【Abstract】 Objective To explore the changes of the serum immune cytokines in medical radiation workers exposure to low dose ionizing radiation. Methods Totally 244 medical professionals working with radiation(61 diagnosis radiology,51 nuclear medicine,74 radio therapeutics and 58 interventional radiology) from 7 hospitals of Guangdong Province were selected as study subjects by using the typical sampling method; 51 administration workers who did not expose to radiation were selected as control group. The radiation dose of these individuals was monitored by thermoluminescent measurement instrument for one year. Venous blood was collected and the levels of interferon γ(IFN-γ),interleukin 10(IL-10),transforming growth factor-β1(TGF-β1) in serum were examined by enzyme-linked immuno sorbent assay. Results The maximum annual average dose of radiation per person of the medical radiation workers was 0. 41 mSv/a. It was smaller than the occupational exposure limit(20. 00 mSv/a). The annual average dose of radiation per person in the group of nuclear medicine was significantly higher than those of diagnosis radiology,radio therapeutics and interventional radiology(P <0. 01). Among the male staffs,the expression of IL-10 in the diagnosis radiology group,radio therapeutics group and interventional radiology group was lower than that in the control group(P < 0. 05); the expression of IL-10 in radio therapeutics group was lower than those in nuclear medicine group and interventional radiology group(P < 0. 05); the ratio of IFN-γ/IL-10 in radio therapeutics group was higher than those in diagnosis radiology group,nuclear medicine group,interventional radiology group and control group(P < 0. 05). These individuals were divided into 3 different dose group(0. 03-,0. 06-and > 0. 15 m Sv/a) based on their average radiation dose. The expression of IL-10 in male staffs of these3 dose groups was lower than that of the male control group(P < 0. 05). Conclusion Long-term low dose ionizing radiation may restrain the expression level of IL-10 in the male staffs.更多还原