【摘要】 目的:研究Aurora-A、B、C(AUR-A、B、C)基因mRNA在急性白血病中的表达情况及其与临床指标的相关性。方法:采用荧光定量PCR检测73例初诊急性白血病(AL)患者(初诊组)、20例化疗完全缓解急性白血病患者(缓解组)和骨髓单个核细胞中AUR-A、B、C mRNA表达水平,14例健康志愿者作为对照(健康组),并分析它们分别在不同分组中的表达性差异,与临床指标的相关性及AUR-A、B、C mRNA表达水平之间的相关性。结果:AURA、B、C mRNA表达在初诊组均高于健康组和缓解组(P<0.01),健康组和缓解组3种基因mRNA表达水平差异均无统计学意义(P>0.05),AUR-A、B、C 3种基因mRNA表达水平在急性淋巴细胞白血病(ALL)组均高于急性髓系白血病(AML)组(P<0.01);AUR-A、B、C mRNA表达在高危组均高于低危组(P<0.05),在高危组与中危组及中危组与低危组之间差异无统计学意义(P>0.05);AUR-A、B、C表达在CD34、CD71、CD56阴性组和阳性组差异无统计学意义(P>0.05),在不同性别、染色体、1疗程化疗是否缓解分组中差异无统计学意义(P>0.05)。73例初诊AL患者中,AUR-A mRNA表达水平与初诊乳酸脱氢酶(LDH)水平和危险分层呈显著正相关(r=0.279,P=0.017;r=0.314,P=0.007);AUR-B与初诊LDH水平和危险分层呈显著正相关(r=0.277,P=0.018;r=0.349,P=0.002),AUR-A、B均与年龄、初诊白细胞(WBC)水平、初诊骨髓幼稚细胞比例和1疗程是否缓解无显著相关性;AUR-C表达水平与初诊白细胞水平、初诊LDH水平和危险分层显著正相关(r=0.263,P=0.025;r=0.348,P=0.003;r=0.376,P=0.001),与年龄显著负相关(r=-0.241,P=0.040),而与初诊骨髓中幼稚细胞比例和1疗程是否缓解无显著相关性;AUR-A和B(r=0.444,P=0.000)、AUR-B和C(r=0.763,P=0.000)、AUR-A和C(r=0.616,P=0.000)mRNA表达水平均呈显著正相关。结论:AUR-A、B、C mRNA在初诊急性白血病患者中高表达并且三者间表达水平显著正相关,其高表达与白血病类型、危险分层、初诊白细胞和乳酸脱氢酶水平等指标相关,均可作为预后指标和潜在治疗靶点更多还原

【Abstract】 Objective: To explore the mRNA expression of Aurora-A,B,C(AUR-A,B,C) in acute leukemia(AL)and their correlations with the clinical indications. Methods: The mRNA expression levels of AUR-A,B,C in 73 cases of newly diagnosed AL(untreated group),20 cases of AL with remission(remission group) and 14 healthy volunteers as control(healthy group) were detected by QRT-PCR,and the difference of expression levels in difference groups,their correlations with clinical indicators and the correlation between the AUR-A,B,C mRNA expression levels themselves were analyzed. Results: The mRNA expression levels of AUR-A,B,C in untreated group were all higher than those in healthy group and remission group(P < 0. 01),but there was not significant difference between healthy group and remission group(P > 0. 05); the mRNA expressions of AUR-A,B,C in acute lymphoblastic leukemia(ALL) group were all significantly higher than that in AML group(P < 0. 01). The mRNA expression of AUR-A,B,C in high risk group was higher than that in low risk group(P < 0. 05),but there was no difference in mRNA expression of AUR-A,B,C be-tween high risk group and middle risk group as well as between middle risk group and low risk group(P > 0. 05). The mRNA expression of AUR-A,B,C in CD34,CD71 and CD56 negative group was not statistically different from that in CD34,CD71 and CD56 positive group(P > 0. 05). In 73 cases of newly diagnosed AL,the mRNA expression levels of AUR-A,B significantly were positively correlated with lactate dehydrogenase(LDH) level and risk stratification(r =0. 279,P = 0. 017; r = 0. 314,P = 0. 007 and r = 0. 277,P = 0. 018; r = 0. 349,P = 0. 002),while the mRNA expression levels of AUR-A,B were not significantly correlated with age,WBC count,blast ratio in bone marrow at initial diagnosis and remission or no-remission after 1 cours of chemotherapy; the mRNA expression level of AUR-C was significantly positively correlated with WBC count(r = 0. 263,P = 0. 025),and LDH level(r = 0. 348,P = 0. 003) at initial diagnosis and risk stratificantion(r = 0. 376,P = 0. 001),and negatively correlated with age(r =-0. 241,P =0. 040),and was not significantly correlated with blast ratio in bone marrow at initial diagnosis and remission or noremission after 1 course of chemotherapy. There were significant positive correlations in the mRNA expression between AUR-A and B(r = 0. 444,P = 0. 000),AUR-B and C(r = 0. 763,P = 0. 000) as well as AUR-A and C(r =0. 616,P = 0. 000). Conclusion: Aur-A,B,C mRNA were highly expressed in patients with newly diagnosed AL,moreover the mRNA expression levels of Aur-A,B,C were positively correlated with each other,the high expression of Aur-A,B,C are associated with leukemia types,risk stratification,WBC count and LDH level at initial diagnosis,so they all maybe used as the prognostic markers and potential therapeutic targets.更多还原