【摘要】 本文介绍了一种非常灵敏并可应用于法医毒物分析的核酸适配体识别技术。这种技术可与多种分析技术手段联用而检测毒物和药物,技术手段包括阻抗滴定技术、表面增强拉曼技术、荧光检测技术、微流控芯片技术、表面等离子共振技术等。核酸适配体技术的检测优势主要表现为选择性强,并且通过纳米金颗粒修饰,可以极大的增强检测信号,实现对痕量目标分子的精准检测。核酸适配体技术由于其易于合成、造价低廉、操作简便、精准识别等优势必将会受到法庭科学家的重视而广泛应用于未来的法医毒物药物分析工作中。更多还原

【Abstract】 In this paper, a novel method was introduced and elucidated for the detection of forensic drugs based on nucleic acid aptamers. The method can be combined with diverse analytical technologies, such as the impedimetry, surfaceenhanced Raman scattering, fluorescent detection, microfludic sensor and the surface-plasmon resonance. The principle of detection is based on an indirect competitive aptamer-assay which can be greatly enhanced its sensitivity to the concentrationlow analytes by resorting to nano-particle coalescence. Aptamers, the molecular recognition agents, are easy to handle for detecting many types of forensically relevant analytes, in particular the drugs. Aptamer-based sensors would fulfill both economical and reliable tasks so that the toxicologists are very hopeful to acquire a time-saving and robust strategic tool for drug analysis.更多还原