【摘要】 目的:建立高效液相色谱法-电喷雾串联质谱联用分析大花罗布麻中植物激素的分析方法.方法:采用C₁₈SPE柱提取大花罗布麻叶中植物激素,在色谱柱为Agilent SB-C₁₈（50 mm×2.1 mm,1.8μm）,0.5%甲酸水-甲醇为流动相,在MRM模式下,ESI负离子模式,毛细管电压为4 k V,干燥器流量为10 L·min^-1,干燥器温度为350℃等条件下,建立液相色谱法-电喷雾串联质谱（LC-MS）联用分析大花罗布麻叶中五种植物激素的方法.结果:大花罗布麻叶中五种植物激素ABA、IBA、IAA、GA₃、JA含量分别为9.800×10³μg·kg^-1、3.073×10⁴μg·kg^-1、1.403×10⁴μg·kg^-1、4.475×10⁴μg·kg^-1、2.508×10⁴μg·kg^-1;五种激素在各自对应的线性范围内线性关系良好.结论:方法灵敏、准确,适合用于大花罗布麻叶中植物激素含量的测定.更多还原

【Abstract】 Objective: To develop a LC-MS method for the determination of plant hormones in Poacyzuan hendersonii. Methods:The plant hormones were isolated and purified by C₁₈ SPE chromatographic techniques. Chromatographic separation was performed on an Agilent SB-C₁₈（ 50 mm × 2. 1 mm,1. 8 μm）. The MS system was operated in the multiple reaction monitoring modes. The MS detection parameters were as follows: the capillary voltage was 4 kV,capillary temperature was 350 ℃,and sheath gas flow rate was 10 L·min^-1. Results: The content of five kinds of plant hormones in the leaves of P. hendersonii were9. 800 × 10³μg·kg^-1,3. 073 × 10⁴μg·kg^-1,1. 403 × 10⁴μg·kg^-1,4. 475 × 10⁴μg·kg^-1,2. 508 × 10⁴μg·kg^-1,respectively. Conclusion: The method is highly sensitive and selective,suitable for the analysis of plant hormones in P. hendersonii.更多还原