【摘要】 为了阐明山羊毕氏肠微孢子虫的遗传多样性,笔者采用基于微卫星(MS1、MS3、MS7)和小卫星(MS4)位点的多位点序列分型技术首次对不同用途山羊的122个毕氏肠微孢子虫分离株进行了多位点序列分型研究。结果发现,在MS1、MS4和MS7位点的扩增效率依次为27.9%(34/122)、18.0%(22/122)、50.8%(62/122),而在MS3位点所有样品均未获得有效扩增。核苷酸序列分析表明,所有样品的MS1、MS4和MS7位点分别具有16、9和18个基因型,共形成了14个MLGs。其中,50份绒山羊阳性样品3个位点扩增效率分别为10.0%(5/50)、14.0%(7/50)和90.0%(45/50),分别具有3、3和10个基因型,共形成了5个MLGs;56份奶山羊阳性样品3个位点的扩增效率依次为28.6%(16/56)、19.6%(11/56)和8.9%(5/56),分别组成4、4、1个基因型,共组成7个不同的MLGs;16份黑山羊阳性样品在3个位点的扩增效率依次为81.3%(13/16)、25.0%(4/16)、75.0%(12/16),分别具有9、2、7个基因型,构成2个MLGs。更多还原

【Abstract】 To indicate the genetic variability of Enterocytozoon bieneusi in goats,the multi-locus sequence genotypes(MLGs)of 122 E.bieneusi isolates from different production categories were studied using the multi-locus sequence genotyping(MLST)technique based on micro-(MS1,MS3,MS7)and mini-satellite(MS4)loci.The results showed that,the respective amplification efficiencies in loci MS1,MS4 and MS7 were 27.9%(34/122),18.0%(22/122),50.8%(62/122),while no amplifications were detected in the locus MS3.The nucleotide sequences analysis indicated that,16,9and 18 genotypes were found in all positive samples for loci MS1,MS4 and MS7,respectively,forming fourteen MLGs.Among them,the amplification efficiencies of three loci among 50 positive samples of cashmere goats were 10.0%(5/50),14.0%(7/50)and 90.0%(45/50),respectively,belonging to three,three and ten genotypes,and five MLGs.The ampli-fication efficiencies of 56 positive samples of dairy goats in three loci were 28.6%(16/56),19.6%(11/56)and 8.9%(5/56),grouping into four,four,one genotypes,and seven different MLGs.The amplification efficiencies of three loci of 16 positive samples of black goats were 81.3%(13/16)and 25.0%(4/16),75.0%(12/16),clustering into 9,2,7genotypes,and two MLGs.更多还原