Objective To report a simple and efficient method to label two different types of retinal ganglion cells(RGCs) in mouse retina.Methods Eyeballs were harvested from normal adult C57 BL/6 J mouse,the retinas were isolated,four radial cuts were done,the retinas were pasted on the nitrocellulose membrane with the ganglion cell layer upturned.The immunofluorescence double staining and laser confocal microscope was used to reveal conventional retinal ganglion cells and intrinsically photosensitive retinal ganglio...
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