【摘要】 目的建立甘扎嘎日药材秀丽莓、红泡刺藤和库页悬钩子中有效成分金丝桃苷的含量测定方法。方法采用HPLC法。在Agela Durashell C₁₈（250mm×4.6mm,5μm）色谱柱上,以乙腈-甲醇-4mL·L^-1磷酸水溶液为流动相,梯度洗脱,检测波长为368nm,柱温为25℃。结果金丝桃苷质量浓度在0.004 0⁰.064 0mg·mL^-1（r=0.999 6）范围内呈良好的线性关系,平均加样回收率为97.35%（RSD=1.09%）。结论该方法操作简便,结果准确、稳定,重复性好,可作为甘扎嘎日药材质量控制和评价的有效方法。更多还原

【Abstract】 Objective To establish an HPLC method for the determination of hyperoside in Kandrakaris such as Rubus amabilis Focke,Rbus niveus Thunb.and Rubus sachalinensia Leveille.Methods The separation was performed on an Agela Durashell C₁₈（250mm×4.6mm,5μm）column with a mobile phase consisting of acetonitrile-methanol-water containing 4mL·L^-1 phosphate in gradient elution.The detection wavelength was set at 368 nm,and the column temperature was 25 ℃.Results The calibration curves were linear within the range of 0.004 0-0.064 0 mg·mL-1（r=0.999 6）for hyperoside.The average recovery was97.35%（RSD=1.09%）.Conclusion The established method for hyperoside in Kandrakarisis simple,the result is accurate,stable and reproducible,and it is acceptable for the quality assurance and evaluation of Kandrakaris.更多还原