Aiming at development of an indirect competitive ELISA(icELISA)kit(Cr-Kit)for measuring total chromium mass concentration in food sample.The method of isothiocyanate(ITC)was used to conjugate Cr~(3+)-iEDTA to bovine serum albumin(BSA)and immunogen Cr~(3+)-iEDTA-BSA was obtained,which was identified by Ultraviolet(UV)and inductively coupled plasma atomic emission spectroscopy(ICP-AES).BALB/c mice were immunized with Cr~(3+)-iEDTA-BSA and hybridoma strains that secrete Cr~(3+)-EDTA mAb were established by cel...
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