【摘要】 以菊花品种‘C008’无菌苗叶片作为外植体,在建立了菊花高效再生体系的基础上,通过农杆菌介导法将CBL基因导入菊花品种‘C008’中。试验共获得抗性植株41株,PCR检测表明,阳性植株为5株,证明CBL基因已转化到菊花‘C008’的基因组DNA中,转化率为12.2%。遗传转化过程中,预培养2d后,将OD₆₀₀=0.5⁰.6的农杆菌稀释50倍后侵染叶片8min,再共培养2d、延迟筛选2d,有利于获得较高的转化率。更多还原

【Abstract】 On the basis of a highly efficient regeneration system of chrysanthemum,the CBLgene was introduced into chrysanthemum ‘C008’by agrobacterium mediated method based on the chrysanthemum‘C008’aseptic seedling leaf as explants.In the experiment,41 strains of resistant plants were obtained.The PCR test showed that the positive plants were 5,which proved that the CBL gene had been transformed into the genome DNA of the chrysanthemum ‘C008’,and the transformation rate was 12.2%.In the process of genetic transformation,after pre-culture for 2d,the explants were infected for 8min with diluted OD₆₀₀=0.5-0.6Agrobacterium culture liquild with 50 times,and then co-cultured for 2d,delayed screening for 2d,which was beneficial to obtain a higher transformation rate.更多还原