【摘要】 目的:检测二肽酶IV(DPP IV)在胃癌组织中的表达,探讨其在胃癌预后中的价值。方法:收集本院手术后的胃癌组织及对应癌旁正常组织112例,用免疫组织化学方法定性比较胃癌组织及癌旁正常胃黏膜组织中DPP IV的表达;Western blot定量比较胃癌组织及癌旁正常组织中DPP IV蛋白的表达。结果:免疫组织化学结果示DPP IV在癌旁正常组织中有3例(占2.7%)表达缺失;胃癌组织中有78例(占69.6%)表达缺失;Western blot结果示DPP IV在胃癌组织中的含量明显低于癌旁正常组织;多因素分析示DPP IV是胃癌不良预后的独立预测因子(P<0.001)。结论:DPP IV表达缺失是胃癌不良预后的独立预测因子。更多还原

【Abstract】 Objective: To evaluate the expression of dipeptidyl peptidase IV(DPP IV) in gastric carcinoma tissue and matched para-tumor tissues, then its clinical pathologic significance for gastric carcinoma was investigated. Methods: One hundred and twelve cases gastric carcinomas tissues and the same number of matched para-tumor tissues were selected, expressive difference of DPP IV gene between carcinoma tissues and their matched para-tumor tissues was measured by immunohistiochemistry; with protein concentration level of DPPIV measured by Western blotting methods. Results: The expressive loss number for DPP IV was 3(2.7%) in 110 normal gastric tissues, while lossed in 78 gastric carcinomas; and the level of DPP IV decreased obviously in DPPIV positive tissues; DPP IV is an independent prognostic factor of gastric carcinoma by multiple factor analysis. Conclusion: Loss of DPP IV’s expression is an independent poor prognostic factor of gastric carcinoma.更多还原