【摘要】 目的:探讨血黏蛋白1(Mucin 1,MUC1)mRNA和细胞角蛋白19片段(cyfra21-1)对非小细胞肺癌(non-small cell lung cancer,NSCLC)的诊断价值。方法:选取80例NSCLC患者(NSCLC组)、50例肺部良性病变患者(良性组)以及40例健康体检者(健康组)为研究对象;检测各组受试人员血MUC1mRNA和cyfra21-1水平,分析MUC1 mRNA和cyfra21-1在NSCLC诊断中的应用价值,比较不同病理特征NSCLC患者血MUC1 mRNA和cyfra21-1阳性率差异。结果:NSCLC组血MUC1 mRNA和cyfra21-1蛋白水平明显高于良性组和健康组(P<0.05),而良性组和健康组血MUC1 mRNA和cyfra21-1水平差异无统计学意义(P>0.05)。MUC1 mRNA诊断NSCLC的最佳临界值为MUC1 mRNA>2.14,其诊断灵敏度为68.8%,特异度为94.4%;血清cyfra21-1最佳临界值为cyfra21-1>3.20 ng/mL,其灵敏度为75.0%,特异度为98.9%。MUC1 mRNA和cyfra21-1联合诊断的灵敏度为87.5%,显著高于MUC1mRNA和cyfra21-1单独检测(P<0.05),而特异度与两者单独检测差异无统计学意义(P>0.05)。血MUC1 mRNA和cyfra21-1表达阳性率与患者肿瘤大小、分化程度、TNM分期及淋巴结转移有关(P<0.05),而与患者年龄、性别以及组织类型无关(P>0.05)。结论:血MUC1 mRNA和cyfra21-1检测有助于NSCLC的临床诊断和病情评估。更多还原

【Abstract】 Objective: To investigate the value of peripheral blood Mucin 1(MUC1) mRNA and cytokeratin 19 fragment(cyfra21-1) in diagnosis of non-small cell lung carcinoma(NSCLC). Methods: Eighty patients with NSCLC(NSCLC group), 50 patients with benign pulmonary disease(benign group) and 40 healthy cases(healthy group)were enrolled in this study. The levels of MUC1 mRNA and cyfra21-1 were detected. The values of MUC1 mRNA and cyfra21-1 in diagnosis of NSCLC were analyzed. The differences of MUC1 mRNA and cyfra21-1 positive ratewere compared in patients with different pathological features. Results: The levels of MUC1 mRNA and cyfra21-1 in NSCLC group were significantly higher than those in the benign group and the healthy group(P<0.05); while there was no significant difference in levels of MUC1 mRNA and cyfra21-1 between the benign group and the healthy group(P>0.05). The best cut-off value of MUC1 mRNA in diagnosis of NSCLC was MUC1 mRNA >2.14 with the sensitivity was 68.8% and specificity was 94.4%. The best cut-off value of cyfra21-1 was cyfra21-1 >3.20 ng/mL with the sensitivity was 75.0% and specificity was 98.9%. The sensitivity of joint application of MUC1 mRNA and cyfra21-1 was 87.5%, which was significantly higher than that of MUC1 mRNA or cyfra21-1 alone(P<0.05), while there was no significant difference in specificity(P>0.05). The positive rates of MUC1 mRNA and cyfra21-1 were significantly correlated with tumor size, differentiated degree, TNM staging, and lymph node metastasis(P<0.05),but no correlated with age, gender and histologic type(P>0.05). Conclusion: The detections of MUC1 mRNA and cyfra21-1 are helpful in clinical diagnosis and disease evaluation of NSCLC.更多还原