【摘要】 目的:观察不同浓度的没食子酸(gallic acid,GA)对鼻咽癌移植荷瘤裸鼠的影响,并探讨其可能的作用机制。方法:建立人鼻咽癌CNE-2细胞株荷瘤裸鼠移植性肿瘤模型,随机分为6组:正常对照组(control,con组),模型组(nasopharynx cancer,NPC组),没食子酸10,20,40μg/kg组(GA-10μg/kg,GA-20μg/kg,GA-40μg/kg)和阳性对照组(顺铂,DDP组);经相应处理后,处死裸鼠,测量瘤体积,称量瘤体重量并计算抑瘤率;流式细胞术检测肿瘤细胞的凋亡率;Western印迹检测肿瘤细胞中cytochrome C(Cyt C)、cleaved-cas 9,cleaved-cas 3与cleaved-PARP的蛋白表达水平。结果:与NPC组相比,给予GA后瘤体体积与重量随其浓度升高而减小,抑瘤率与肿瘤细胞凋亡率增大;与NPC组相比,肿瘤细胞线粒体中Cyt C的蛋白表达随GA浓度增加而减少,胞质中CytC的蛋白表达则相反,细胞中cleaved-cas 9,cleaved-cas 3与cleaved-PARP的蛋白水平显著增加,与GA浓度呈正相关。结论:没食子酸可能通过调控线粒体通路诱导鼻咽癌移植瘤细胞的凋亡。更多还原

【Abstract】 Objective: To investigate the effects of gallic acid(GA) on the xenograft model of nasopharynx cancer in nude mice and to study its possible mechanism. Methods: Xenograft model of human nasopharynx cancer was established by transplantation of human nasopharynx cancer cell CNE-2. The mice were divided into 6 groups: the control group, the nasopharynx cancer group, the galllic acid(10 μg/kg) group, the galllic acid(20 μg/kg) group, the galllic acid(40 μg/kg) group and the DDP group. The tumor dimensions and volume were measured and the tumor inhibiting rate was calculated. The apoptotic rate of CNE-2 cells was examined with flow cytometry. The protein levels of cytochrome C, cleaved-cas 9, cleaved-cas 3 and cleaved-PARP were detected by Western blotting. Results: Compared with the nasopharynx cancer group, the tumor volume and weight significantly decreased in the galllic acid group, and the inhibitory rate and apoptotic rate of the tumor dose-dependently increased. Moreover, galllic acid enhanced the release of cytochrome C from mitochondria to cytoplasm, leading to increase the protein levels of cleaved-cas 9, cleaved-cas 3 and cleaved-PARP. Conclusion: Galllic acid induces apoptosis of nasopharynx cancer cell CNE-2 through mitochondria-dependent pathway.更多还原