Aflatoxin B1 artificial antigen( AFB1-BSA) was prepared with EDC method. The results showed that the UV spectrum of AFB1-BSA was different from BSA and Aflatoxin B1 obviously,and the IV spectrum was also different from BSA distinctly,all of which indicated that the preparation of AFB1-BSA was successful. BALB/c mice were immunized wth different molar ratio of AFB1-BSA artificial antigen,which made preparation of the specific polyclonal antiserum against AFB1. This paper comprehensively evaluated the immune ...
黄曲霉毒素B1(Aflatoxin B1,简称AFB1)是由黄曲霉(Aspergillus flavus)和寄生曲霉(Asper-gillus parasiticus)产生的主要次级代谢产物之一,是目前所发现的一类强毒性,强致畸致癌性的真菌毒素(Ono et al.,2001;冯广鹏等,2007;黄向华等,2004;Busby et al.,1984;马健等,2012)。在?